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Showing papers in "Plant Physiology and Biochemistry in 2005"


Journal ArticleDOI
TL;DR: The present data indicate that Cadmium, like other metals, induces cellular redox disequilibrium suggesting that an environmentally realistic concentration of cadmium can cause oxidative stress.

315 citations


Journal ArticleDOI
TL;DR: Simultaneous monitoring of short term and long term stress acclimatization showed evidence that the metabolic strategies to cope with increasing nutrient limitation are highly species-specific.

279 citations


Journal ArticleDOI
TL;DR: The results clearly indicate the protective role of Zn in modulating the redox status of the plant system through the antioxidant pathway AGC and GSH metabolic enzymes for combating Cd induced oxidative stress.

272 citations


Journal ArticleDOI
TL;DR: Results provide the first indication that temperature is an important environmental factor to optimize the secondary metabolite production in St. John's wort and controlled environment technology can allow the precise application of such specific stresses.

245 citations


Journal ArticleDOI
Robert D. Slocum1
TL;DR: Gene expression profiling for pathway enzymes further suggests that NAGS, NAGK, NAOGAcT and NAOD are coordinately regulated in response to changes in Arg demand during plant growth and development.

245 citations


Journal ArticleDOI
TL;DR: In this paper, the thermal dependencies of the activities of protective enzymes, photosynthetic efficiency (Fv/Fm), protein, non-protein thiol (NP-SH), cysteine content, lipoxygenase (LOX) activity (EC 1.13.5.12), and malondialdehyde (MDA) content at 25-40 degrees C were determined for 4, 24 and 48 h in leaf and root segments of Phalaenopsis.

205 citations


Journal ArticleDOI
TL;DR: It is suggested that cGAPDH in Arabidopsis might also have such a role in mediating ROS signalling in plants, as well as being inactivated by H2O2 in vitro and reversible by the subsequent addition of reductants such as reduced glutathione (GSH).

204 citations


Journal ArticleDOI
TL;DR: This critical review focuses on the characterization of most plant serine proteases, and comprehensively lists information produced by more and more sophisticated research tools that have led to the current state of the art in knowledge of these unique enzymes.

184 citations


Journal ArticleDOI
TL;DR: The results suggested that the decreased yield in pumpkins was related to the UV-B impaired flow of electrons in the respiratory chain, and no significant effect of excludingUV-B radiation or of treatment with selenium was observed on the photochemical efficiency of photosystem II (PSII).

165 citations


Journal ArticleDOI
TL;DR: In this paper, the role of ZIP and NRAMP transporters for Ni2+-transport and homeostasis was investigated by expression in yeast, and the results indicated that ZIP/NRAMP-transporters participate in Ni2-homeostasis of Ni hyperaccumulator plants.

147 citations


Journal ArticleDOI
TL;DR: Following the growth of transgenic and non- transformed canola in nickel contaminated soil, it was observed that the rolD plants demonstrate significantly increased tolerance to nickel compared to the non-transformed control plants.

Journal ArticleDOI
TL;DR: This work shows for the first time that a transcriptional regulation of PM H(+)-ATPase involving a specific isoform occurs in the response to Fe deficiency, and appears to be unaffected by Fe.

Journal ArticleDOI
TL;DR: The effects of elevated atmospheric hydrogen sulfide levels on the model plant Arabidopsis thaliana (L.) Heynh and the molecular basis for the increased thiol levels, enzyme activities, messenger RNA and protein steady-state levels of cysteine-synthesizing and degrading pathways have been determined.

Journal ArticleDOI
TL;DR: Results indicate that in leaves the stress generated by the low accumulation of Cd seems to be counteracted by the antioxidant response and by the PC biosynthesis, and in roots, in spite of the elevated presence of PCs and related thiol-peptide-compounds, the excess ofCd causes a decline in the antioxidant protection of the organ, with the consequent generation of considerable amounts of H(2)O(2), a direct agent of oxidative stress.

Journal ArticleDOI
TL;DR: The results indicate that PDAT activity encoded by At5g13640 is not a major determinant of TAG synthesis in Arabidopsis seeds, a contrary situation to yeast where PDAT is a major contributor to triacylglycerol accumulation in exponential growth phase.

Journal ArticleDOI
TL;DR: Real-time PCR assays based on TaqMan chemistry have been developed and are particularly suited to the detection and quantification of the pathogen prior to the development of symptoms, and should be ideally suited to investigating infection processes in-planta.

Journal ArticleDOI
TL;DR: The fraction of unidentified complex sphingolipids, containing mainly 8E-phytosphingenine, exceeds the cerebroside proportion in PMs by several factors and may be as abundant as diacylglycerol-based phospholIPids.

Journal ArticleDOI
TL;DR: The results suggest that soluble sugars are involved in the mechanism of resistance, as they can stimulate phenylpropanoid metabolism and contribute to the increase in concentration of isoflavonoids, which are important elements of the defense system of legumes.

Journal ArticleDOI
TL;DR: The vacuolar H+ -PPase pump the authors cloned (TVP1) is the first member of this gene family cloned from wheat and deduced translation product is homologous to proteins encoded by genes in barley, rice, and Arabidopsis.

Journal ArticleDOI
TL;DR: In this paper, a rice root was fed with AsA and oxalate and its putative precursors to observe the resistance of rice to chilling, water stress, and toxicity.

Journal ArticleDOI
TL;DR: The results indicate that the glycyrrhizin concentrations of 3-6 months old pot plants were similar or even higher than the previously reported values for 3-4 years old field-grown plants and confirm that high concentration of Glycyr rhizin production is possible within a very short production period under controlled environments.

Journal ArticleDOI
TL;DR: It is shown that the key feature of the adsorption is globally preserved: aromatic rings of lignin adopt a preferential parallel orientation relative to the cellulose surface, which rationalize the apparent contradiction between the available experimental results.

Journal ArticleDOI
TL;DR: The regulatory properties and gene expression of phytoene synthase (PSY), a key enzyme involved in carotenoid biosynthesis, was analyzed in tomato fruit, suggesting that PSY may be subject to post-translational regulation.

Journal ArticleDOI
TL;DR: This research investigated ROS and NO generation in tomato plants treated with o-hydroxyethylorutin, non-treated and infected ones and found the elevated NO concentration in tomato leaves strongly decreased hydrogen peroxide concentration without affecting other studied ROS levels.

Journal ArticleDOI
TL;DR: The results presented here show that SEN1 is regulated by signals that link plant defence and senescence responses and thus represents a useful marker gene to study the overlap between these two important physiological events.

Journal ArticleDOI
TL;DR: It appeared that myrosinase from horseradish root consists of two subunits of similar molecular mass of about 65 kDa, which was able to breakdown intact glucosinolates in a crude extract of broccoli.

Journal ArticleDOI
TL;DR: In this paper, the assembly of cellulose and lignin was investigated at the atomistic scale using molecular dynamics simulations, and the dynamical process of adsorption of the Lignin dimer onto the different surfaces of the cellulose crystal was examined.

Journal ArticleDOI
TL;DR: Ginseng rusty root, a disorder of unknown cause (s), in which reddish-brown to orange-brown areas develop on the surface of field-grown roots, was studied at the cellular and biochemical levels as mentioned in this paper.

Journal ArticleDOI
TL;DR: This is the first biochemical evidence that phenotypic changes in plant cells induced by DNA hypo- or hypermethylating treatments are correlated in a linear relationship to modifications of the cell wall differentiation state.

Journal ArticleDOI
TL;DR: It is concluded that softening during ripening in banana fruit results from the concerted action of at least four PG genes, which are differentially expressed during ripens.