Journal ArticleDOI
Benzophenone photophores in biochemistry
György Dormán,Glenn D. Prestwich +1 more
TLDR
This Perspectives includes a brief review of BP photochemistry and a selection of specific applications of these photoprobes, which address questions in protein, nucleic acid, and lipid biochemistry.Abstract:
The photoactivatable aryl ketone derivatives have been rediscovered as biochemical probes in the last 5 years. The expanding use of benzophenone (BP) photoprobes can be attributed to three distinct chemical and biochemical advantages. First, BPs are chemically more stable than diazo esters, aryl azides, and diazirines. Second, BPs can be manipulated in ambient light and can be activated at 350-360 nm, avoiding protein-damaging wavelengths. Third, BPs react preferentially with unreactive C-H bonds, even in the presence of solvent water and bulk nucleophiles. These three properties combine to produce highly efficient covalent modifications of macromolecules, frequently with remarkable site specificity. This Perspectives includes a brief review of BP photochemistry and a selection of specific applications of these photoprobes, which address questions in protein, nucleic acid, and lipid biochemistry.read more
Citations
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Journal ArticleDOI
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
TL;DR: An orthogonal aminoacyl-tRNA synthetase/tRNA pair is evolved that makes possible the in vivo incorporation of p-benzoyl-l-phenylalanine into proteins in Escherichia coli in response to the amber codon, TAG.
Journal ArticleDOI
Chemical cross-linking and mass spectrometry to map three-dimensional protein structures and protein-protein interactions.
TL;DR: The most popular cross-linking reagents for protein structure analysis are described and an overview of the different available strategies that employ chemical cross- linking and different mass spectrometric techniques are given.
Journal ArticleDOI
Skin-inspired hydrogel-elastomer hybrids with robust interfaces and functional microstructures
TL;DR: Zhao et al. as discussed by the authors proposed a simple yet versatile method to assemble hydrogels and elastomers into hybrids with extremely robust interfaces (interfacial toughness over 1,000 Jm−2) and functional microstructures such as microfluidic channels and electrical circuits.
Journal ArticleDOI
Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light.
David A. Fancy,Thomas Kodadek +1 more
TL;DR: The de novo design and development of a type of protein cross-linking reaction that uses a photogenerated oxidant to mediate rapid and efficient cross- linking of associated proteins.
Journal ArticleDOI
Using photolabile ligands in drug discovery and development.
György Dormán,Glenn D. Prestwich +1 more
TL;DR: Examples are presented from three fundamentally different approaches: (1) photoaffinity labeling of target macromolecules; (2) photoactivation and release of 'caged ligands'; and (3) photoimmobilization of ligands onto surfaces.
References
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Journal ArticleDOI
Multiplexed biochemical assays with biological chips
Stephen P. A. Fodor,Richard P. Rava,Xiaohua C. Huang,Ann Maria Caviani Pease,Christopher P. Holmes,Cynthia L. Adams +5 more
TL;DR: High density peptide and oligonucleotide chips are fabricated using semiconductor-based technologies and have a variety of biological applications.
Journal ArticleDOI
New Photolabeling and Crosslinking Methods
TL;DR: The author revealed that photo-activatable Phospholipids as Photo-affinity Probes as well as the properties of Reactive Amino Acid Residues and Photolabeling of Membranes had changed in the last decade.
Journal ArticleDOI
Biomimetic control of chemical selectivity
TL;DR: A number of enzyme mimics have been constructed to imitate an enzymatic mechanism, using the same catalytic groups which the enzyme uses and examining the question of whether a proposed mechanism for the enzyme process is reasonable or duplicable as discussed by the authors.
Book ChapterDOI
Biomimetic control of chemical selectivity
TL;DR: One of the challenges for enzyme mimics is to duplicate not only the mechanism by which the enzyme operates but also the very large rate of the process.
Journal ArticleDOI
p-Benzoyl-L-phenylalanine, a new photoreactive amino acid. Photolabeling of calmodulin with a synthetic calmodulin-binding peptide.
TL;DR: A new photoreactive amino acid analog, p-benzoyl-L-phenylalanine, is described and its utility was illustrated in place of tryptophan in a 17-residue calmodulin-binding peptide.