Journal ArticleDOI
Biocalorimetric and respirometric studies on production of Penicillin G acylase from Bacillus badius pac in E. coli DH5α
Rajendran Karthikeyan,Mahadevan Surianarayanan,Sekar Sudharshan,Paramasamy Gunasekaran,Mandal Asit Baran +4 more
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TLDR
Metabolic heat flow rate profile and heat yields at different levels helped to conclude that the recombinant construct pPROPAC was an obligate aerobic, and can contribute to scale up studies, possibly.About:
This article is published in Biochemical Engineering Journal.The article was published on 2011-08-15. It has received 16 citations till now. The article focuses on the topics: Bacillus badius & Respirometry.read more
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Biotechnological advances on penicillin G acylase: pharmaceutical implications, unique expression mechanism and production strategies.
TL;DR: A state-of-the-art review in recent biotechnological advances associated with PGA, particularly in the production technologies with an emphasis on using the Escherichia coli expression platform.
Journal ArticleDOI
Microbial Biotransformation to Obtain New Antifungals.
Luiz F. Bianchini,Maria Fernanda Cordeiro Arruda,Sérgio Vieira,Patrícia Maria Stuelp Campelo,Ana Maria Trindade Grégio,Edvaldo Antonio Ribeiro Rosa +5 more
TL;DR: This review was conceived to present how Pharmaceutical Biotechnology might contribute to the discovery of molecules with antifungal properties by microbial biotransformation procedures, which aims the highest possible efficiency with reduced production of waste and the smallest environmental impact.
Journal ArticleDOI
Biodegradation of tannery dye effluent using Fenton's reagent and bacterial consortium: A biocalorimetric investigation.
Bhuvanesh Kumar Shanmugam,Sivanesh Nanjan Easwaran,Anusha Subramanian Mohanakrishnan,Chitra Kalyanaraman,Surianarayanan Mahadevan +4 more
TL;DR: The study explores a hybrid method of combining Fenton oxidation with biological treatment by a defined bacterial consortium for the biodegradation of an effluent containing toxic azo dye.
Journal ArticleDOI
High-throughput strategies for penicillin G acylase production in rE. coli fed-batch cultivations
A. M. Velez,Adilson José da Silva,Antonio Carlos Luperni Horta,Cintia Regina Sargo,Gilson Campani,G. G. Silva,Raquel L. C. Giordano,Teresa Cristina Zangirolami +7 more
TL;DR: The complex medium was the most cost-effective for PGA production, and could be used in both high cell density and straightforward auto-induction protocols.
Journal ArticleDOI
Bioenergetics and pathway of acid blue 113 degradation by Staphylococcus lentus.
TL;DR: The degradative pathway for acid blue 113 by S. lentus was delineated via high‐performance liquid chromatography, Fourier transform infrared spectroscopy, and gas chromatography coupled with mass spectrometry analyses, and Interestingly the products of degradation were found to have low toxicity, as per cytotoxicity measurements.
References
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Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Book
Biochemical engineering fundamentals
James E. Bailey,David F. Ollis +1 more
TL;DR: Biochemical Engineering Fundamentals, 2/e as mentioned in this paper combines contemporary engineering science with relevant biological concepts in a comprehensive introduction to biochemical engineering, which enables students to comprehend the major problems in biochemical engineering and formulate effective solutions.
Book
Bioprocess Engineering: Basic Concepts
TL;DR: The author explains how the biotechnology and bioprocess engineering profession has changed in the modern era, and some examples of this change can be found in the chapters on Bioreactors for Suspension and Immobilized Cultures.
Journal ArticleDOI
Industrial production of β-lactam antibiotics
TL;DR: The industrial production of beta-lactam antibiotics by fermentation over the past 50 years is one of the outstanding examples of biotechnology as mentioned in this paper, and major improvements in the productivity of the producer organisms, Penicillium chrysogenum (syn. Cephalosporium acremonium) and improved fermentation technology have culminated in enhanced productivity and substantial cost reduction.
Journal ArticleDOI
The isolation and kinetics of penicillin amidase from Escherichia coli
TL;DR: The enzyme was shown to be inhibited by excess substrate, benzylpenicillin, and by both of the products of hydrolysis, which was found to be competitive and by 6-aminopenicillanic acid to be non-competitive.
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