Journal ArticleDOI
Biotechnological enhancement of capsaicin biosynthesis in cell suspension cultures of Naga King Chili ( Capsicum chinense Jacq.)
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Addition of the calcium channel blocker verapamil hydrochloride inhibited cell growth and capsaicin production in Naga King Chili suspension cell cultures.Abstract:
Cell suspension cultures were initiated from hypocotyl derived callus to induce capsaicin biosynthesis in suspension cultures of Naga King Chili (Capsicum chinense Jacq.). Efficient capsaicin production with high growth index (GI) was obtained by exposing cells to salicylic acid (SA) and calcium channel modulators in suspension cultures. The time course of capsaicin formation is related to the cell growth profile in a batch culture. Cells cultivated in the standard medium (SM) initially showed low level of capsaicin yield during active growth. When the cells approached stationary phase, cell growth and cell viability decreased whereas capsaicin production increased continuously. In the fed-batch cultures, the highest capsaicin yield (567.4 ± 8.1 μgg(1) fresh weight) (f.wt) was obtained by feeding the cells with 1 mM SA. However, SA feeding during cultivation repressed the cell growth. Enhanced cell growth (3.1 ± 0.1 GI/culture) and capsaicin yield (534 ± 7.8 μgg(-1)f.wt) were obtained when the cells were fed with calcium ionophore A23187 (0.5 mM) on day 25 as compared to the control. Addition of the calcium channel blocker verapamil hydrochloride (100 mM) inhibited cell growth and capsaicin production in Naga King Chili suspension cell cultures.read more
Citations
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Journal ArticleDOI
Chemical elicitors versus secondary metabolite production in vitro using plant cell, tissue and organ cultures: recent trends and a sky eye view appraisal
C. C. Giri,Mohd Zaheer +1 more
TL;DR: Elicitors singly with media additives, combination of elicitors and elicitors other than signal compounds along with hormones were found beneficial for enhanced secondary metabolite production and a combination of target gene over expression and elicitor treatment supported higher secondary product yield.
Journal ArticleDOI
Biochemistry and molecular biology of capsaicinoid biosynthesis: recent advances and perspectives
TL;DR: Advances made by different researchers since a previous review of the biochemistry and molecular biology of capsaicinoid biosynthesis are described, including the contribution of omics to the knowledge of this pathway.
Journal ArticleDOI
Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field.
Raffaella Balestrini,Cecilia Brunetti,Maria Cammareri,Sofia Caretto,Valeria Cavallaro,Eleonora Cominelli,Monica De Palma,Teresa Docimo,Giovanna Giovinazzo,Silvana Grandillo,Franca Locatelli,Erica Lumini,Dario Paolo,Cristina Patanè,Francesca Sparvoli,Marina Tucci,Elisa Zampieri +16 more
TL;DR: In this article, a review of Mediterranean crop plants as a source of specialized metabolites is presented, with a special attention on the strategies that can be used to modulate their production, including abiotic stresses, interaction with beneficial soil microorganisms and novel genetic approaches.
Book ChapterDOI
Conservation and sustainable use of medicinal plants
TL;DR: This chapter oversees this and gave insight on the various propagation processes for conservation through efficient biotechnological processes such as cell and tissue culture amplification of bioactive compounds of interests in vitro.
References
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
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Journal Article
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