Book ChapterDOI
Bone Marrow Preparations for Chromosome Studies
T. C. Hsu,T. C. Hsu,James L. Patton,James L. Patton +3 more
- pp 454-460
TLDR
Metaphase chromosome preparations from primary cultures are usually of better quality than those from bone marrow, but in a number of cases bone marrow is the only practical material for cytological preparations; therefore, mastering this technique is essential in studies of mammalian karyotypes.Abstract:
Metaphase chromosome preparations from primary cultures are usually of better quality than those from bone marrow. However, in a number of cases bone marrow is the only practical material for cytological preparations; therefore, mastering this technique is essential in studies of mammalian karyotypes. These reasons include:
1
Not every investigator has a tissue culture laboratory at his disposal.
2
Direct bone marrow preparations reduce the cost.
3
In field trips, bone marrows can be processed with relative ease without large pieces of equipment and supply items, such as fresh growth media.
4
Bone marrow preparations should be made as an insurance in case primary cultures fail or become contaminated.
5
In case of mosaicism, bone marrow offers an excellent second material.read more
Citations
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Journal ArticleDOI
Loss of telomeric sites in the chromosomes of Mus musculus domesticus (Rodentia:Muridae) during Robertsonian rearrangements.
TL;DR: The elimination of substantial amounts of chromosomal DNA during the formation of mouse Rb chromosomes is demonstrated, indicating that the breakpoints required for a Robertsonian process do not include telomeric sites exclusively but extend to the adjacent pericentromeric regions of the original acrocentric chromosomes.
Journal ArticleDOI
Pattern of activity of nucleolus organizers during spermatogenesis in mammals as analyzed by silver-staining.
TL;DR: Silver-staining in the nuclei and chromosomes of spermatogenesis of four species of mammals was investigated qualitatively and quantitatively and reveals minor differences between the species investigated with regard to the times and extents of maximum activation.
Journal ArticleDOI
Genetic monitoring detects an overlooked cryptic species and reveals the diversity and distribution of three invasive Rattus congeners in south Africa
Armanda D.S. Bastos,Armanda D.S. Bastos,Deenadayalan Nair,Peter J. Taylor,Helene Brettschneider,F. Kirsten,El-Marie Mostert,El-Marie Mostert,Emil Von Maltitz,Jennifer M. Lamb,Pim van Hooft,Pim van Hooft,Steven R. Belmain,G. Contrafatto,Sarah Downs,Christian Timothy Chimimba,Christian Timothy Chimimba +16 more
TL;DR: This study has highlighted the value of genetic typing for detecting cryptic invasive species, providing historical insights into introductions and for directing future sampling, and indicated that R. rattus probably became established following at least two and three independent introductions, respectively.
Journal ArticleDOI
Karyotype and genetic evolution in speciation of subterranean mole rats of the genus Spalax in Turkey
Eviatar Nevo,Maria Grazia Filippucci,Carlo Alberto Redi,Shimon Simson,Giora Heth,Avigdor Beiles +5 more
TL;DR: Climatic selection in Turkey appears to be a major architect of karyotype and genetic diversity and divergence in mole rat evolution, in both speciation and adaptation, and support the niche-width genetic variation hypothesis in space and time.
Journal ArticleDOI
Contact zone between chromosomal races of Mus musculus domesticus. 2. Fertility and segregation in laboratory-reared and wild mice heterozygous for multiple robertsonian rearrangements.
TL;DR: Litter size, anaphase I nondisjunction and X–Y dissociation at metaphase I were studied in homozygous and heterozygous house mice from a central Italian chromosomal hybrid zone and the stability of this hybrid zone through time is discussed.