Journal ArticleDOI
Bone Tissue Engineering using human mesenchymal stem cells: effects of scaffold material and medium flow
Lorenz Meinel,Vassilis Karageorgiou,Robert Fajardo,Brian D. Snyder,Vivek R. Shinde-Patil,Ludwig Zichner,David L. Kaplan,Robert Langer,Gordana Vunjak-Novakovic +8 more
TLDR
It is suggested that osteogenesis in cultured MSC can be modulated by scaffold properties and flow environment.Abstract:
We report studies of bone tissue engineering using human mesenchymal stem cells (MSCs), a protein substrate (film or scaffold; fast degrading unmodified collagen, or slowly degrading cross-linked collagen and silk), and a bioreactor (static culture, spinner flask, or perfused cartridge). MSCs were isolated from human bone marrow, characterized for the expression of cell surface markers and the ability to undergo chondrogenesis and osteogenesis in vitro, and cultured for 5 weeks. MSCs were positive for CD105/endoglin, and had a potential for chondrogenic and osteogenic differentiation. In static culture, calcium deposition was similar for MSC grown on collagen scaffolds and films. Under medium flow, MSC on collagen scaffolds deposited more calcium and had a higher alcaline phosphatase (AP) activity than MSC on collagen films. The amounts of DNA were markedly higher in constructs based on slowly degrading (modified collagen and silk) scaffolds than on fast degrading (unmodified collagen) scaffolds. In spinner flasks, medium flow around constructs resulted in the formation of bone rods within the peripheral region, that were interconnected and perpendicular to the construct surface, whereas in perfused constructs, individual bone rods oriented in the direction of fluid flow formed throughout the construct volume. These results suggest that osteogenesis in cultured MSC can be modulated by scaffold properties and flow environment.read more
Citations
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Journal ArticleDOI
Silk as a biomaterial
Charu Vepari,David L. Kaplan +1 more
TL;DR: Silks are fibrous proteins with remarkable mechanical properties produced in fiber form by silkworms and spiders that are biocompatible when studied in vitro and in vivo.
Journal ArticleDOI
Bone Tissue Engineering: Recent Advances and Challenges
TL;DR: The fundamentals of bone tissue engineering are discussed, highlighting the current state of this field, and the recent advances of biomaterial and cell-based research, as well as approaches used to enhance bone regeneration.
Journal ArticleDOI
Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends
João F. Mano,Gabriel A. Silva,Helena S. Azevedo,P. B. Malafaya,Rui A. Sousa,Simone S. Silva,Luciano F. Boesel,Joaquim M. Oliveira,Tírcia C. Santos,Alexandra P. Marques,Nuno M. Neves,Rui L. Reis +11 more
TL;DR: In this review, the most studied and promising and recently proposed naturally derived polymers that have been suggested for tissue engineering applications are described and their blends with synthetic polymers are analysed, with special focus on polysaccharides and proteins.
Journal ArticleDOI
Stem cell-based tissue engineering with silk biomaterials.
TL;DR: Stem cell-based tissue engineering using 3D silk fibroin scaffolds has expanded the use of silk-based biomaterials as promising scaffolds for engineering a range of skeletal tissues like bone, ligament, and cartilage, as well as connective tissues like skin.
Journal ArticleDOI
Polymeric Materials for Bone and Cartilage Repair
TL;DR: Current strategies in scaffold-guided tissue engineering approach, involving the most employed biodegradable polymers, either of natural or synthetic origin, will be reported underlying the role played by both material structure–property relationship and scaffold architecture.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue
TL;DR: A modified form of the dim methylmethylene blue assay is described that has improved specificity for sulphated glycosaminoglycans, and it is shown that in conjunction with specific polysaccharidases, the dimethylmethyleneblue assay can be used to quantitate individual sulphated sugarcans.
Journal ArticleDOI
Purification and Characterization of Mouse Hematopoietic Stem Cells
TL;DR: Mouse bone marrow hematopoietic stem cells were isolated with the use of a variety of phenotypic markers and thirty of these cells are sufficient to save 50 percent of lethally irradiated mice, and to reconstitute all blood cell types in the survivors.
Journal ArticleDOI
Tissue-engineered bone regeneration.
Hervé Petite,Véronique Viateau,Wassila Bensaïd,Alain Meunier,Cindy de Pollak,Marianne Bourguignon,Karim Oudina,Laurent Sedel,Genevieve Guillemin +8 more
TL;DR: A combination of a coral scaffold with in vitro-expanded marrow stromal cells (MSC) to increase osteogenesis more than that obtained with the scaffold alone or the scaffolds plus fresh bone marrow, and thus improve clinical outcome.