c-Type cytochrome-dependent formation of U(IV) nanoparticles by Shewanella oneidensis.
Matthew J. Marshall,Alexander S. Beliaev,Alice Dohnalkova,David W. Kennedy,Liang Shi,Zheming Wang,Maxim I. Boyanov,Barry Lai,Kenneth M. Kemner,Jeffrey S. McLean,Samantha B. Reed,David E. Culley,Vanessa L. Bailey,Cody J. Simonson,Daad A. Saffarini,Margaret F. Romine,John M. Zachara,James K. Fredrickson +17 more
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TLDR
It is shown that c-type cytochromes of a dissimilatory metal-reducing bacterium, Shewanella oneidensis MR-1, are essential for the reduction of U(VI) and formation of extracelluar UO 2 nanoparticles and this is the first study to the authors' knowledge to directly localize the OM-associated cyto Chromes with EPS, which contains biogenic UO2 nanoparticles.Abstract:
Modern approaches for bioremediation of radionuclide contaminated environments are based on the ability of microorganisms to effectively catalyze changes in the oxidation states of metals that in turn influence their solubility. Although microbial metal reduction has been identified as an effective means for immobilizing highly-soluble uranium(VI) complexes in situ, the biomolecular mechanisms of U(VI) reduction are not well understood. Here, we show that c-type cytochromes of a dissimilatory metal-reducing bacterium, Shewanella oneidensis MR-1, are essential for the reduction of U(VI) and formation of extracelluar UO 2 nanoparticles. In particular, the outer membrane (OM) decaheme cytochrome MtrC (metal reduction), previously implicated in Mn(IV) and Fe(III) reduction, directly transferred electrons to U(VI). Additionally, deletions of mtrC and/or omcA significantly affected the in vivo U(VI) reduction rate relative to wild-type MR-1. Similar to the wild-type, the mutants accumulated UO 2 nanoparticles extracellularly to high densities in association with an extracellular polymeric substance (EPS). In wild-type cells, this UO 2-EPS matrix exhibited glycocalyx-like properties and contained multiple elements of the OM, polysaccharide, and heme-containing proteins. Using a novel combination of methods including synchrotron-based X-ray fluorescence microscopy and high-resolution immune-electron microscopy, we demonstrate a close association of the extracellular UO 2 nanoparticles with MtrC and OmcA (outer membrane cytochrome). This is the first study to our knowledge to directly localize the OM-associated cytochromes with EPS, which contains biogenic UO 2 nanoparticles. In the environment, such association of UO 2 nanoparticles with biopolymers may exert a strong influence on subsequent behavior including susceptibility to oxidation by O 2 or transport in soils and sediments.read more
Citations
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Biological synthesis of metallic nanoparticles.
TL;DR: This review provides an overview of various methods of synthesis of metallic nanoparticles by biological means and indicates that a promising approach to achieve this objective is to exploit the array of biological resources in nature.
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Biological synthesis of metal nanoparticles by microbes.
TL;DR: The current status of microbial synthesis and applications of metal nanoparticles is presented and several factors such as microbial cultivation methods and the extraction techniques have to be optimized and the combinatorial approach such as photobiological methods may be used.
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‘Green’ synthesis of metals and their oxide nanoparticles: applications for environmental remediation
TL;DR: This review summarized the fundamental processes and mechanisms of “green” synthesis approaches, especially for metal and metal oxide nanoparticles using natural extracts and explored the role of biological components, essential phytochemicals (e.g., flavonoids, alkaloids, terpenoids, amides, and aldehydes) as reducing agents and solvent systems.
Journal ArticleDOI
Respiration of metal (hydr)oxides by shewanella and geobacter: a key role for multihaem c-type cytochromes
TL;DR: Multihaem c‐Cyts play critical roles in S. oneidensis MR‐1‐ and G.’sulfurreducens‐mediated dissimilatory reduction of solid metal (hydr)oxides by facilitating ET across the bacterial cell envelope.
Journal ArticleDOI
Current Production and Metal Oxide Reduction by Shewanella oneidensis MR-1 Wild Type and Mutants
Orianna Bretschger,Anna Obraztsova,Carter A. Sturm,In Seop Chang,In Seop Chang,Yuri A. Gorby,Samantha B. Reed,David E. Culley,Catherine L. Reardon,Soumitra Barua,Soumitra Barua,Margaret F. Romine,Jizhong Zhou,Jizhong Zhou,Alexander S. Beliaev,Rachida Bouhenni,Daad A. Saffarini,Florian Mansfeld,Byung Hong Kim,Byung Hong Kim,James K. Fredrickson,Kenneth H. Nealson +21 more
TL;DR: The results showed that a few key cytochromes play a role in all of the processes but that their degrees of participation in each process are very different, suggesting a very complex picture of electron transfer to solid and soluble substrates by S. oneidensis MR-1.
References
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