Journal ArticleDOI
Callus formation from cell culture protoplasts of corn (Zea mays L.).
TLDR
Routine procedures for the isolation of large numbers of protoplasts from an established cell culture of Zea mays and for the induction of sustained divisions leading to secondary cell cultures have been developed.Abstract:
Routine procedures for the isolation of large numbers of protoplasts from an established cell culture of Zea mays and for the induction of sustained divisions leading to secondary cell cultures have been developed. The critical factors seem to be associated with neither specific enzymatic conditions for the isolation nor specific culture conditions for the protoplasts but with the ‘quality’ of the culture used for protoplast isolation.read more
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Patent
Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof
Thomas R. Adams,Sheryl A. Chambers,Richard J. Daines,William J. Gordon-Kamm,Albert P. Kausch,Peggy G. Lemaux,Catherine J. Mackey,Mary L. Mangano,James V. O'brien,Thomas B. Rice,T. Michael Spencer,William G. Start,Nancy Willetts +12 more
TL;DR: In this article, a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed, is described. But the system is not applicable to the field of agriculture.
PatentDOI
Fertile transgenic corn plants
TL;DR: Fertile transgenic Zea mays (corn) plants which stably express recombinant DNA which is heritable are provided wherein said DNA preferably comprises a recombinant gene which encodes a seed storage protein, so that the amino acid profile of the corn is improved as discussed by the authors.
Journal ArticleDOI
Agrobacterium -mediated transformation of rice using immature embryos or calli induced from mature seed
Yukoh Hiei,Toshihiko Komari +1 more
TL;DR: Comprehensive, highly efficient protocols for Agrobacterium tumefaciens-mediated transformation of a wide range of rice genotypes are provided.
Journal ArticleDOI
Developing Cell and Tissue Culture Systems for the Improvement of Cereal and Grass Crops
TL;DR: The cereals and grasses, which constitute the most important group of crop plants, have until recently been found to be very recalcitrant to cell culture techniques, and the advances made in cell culture of important members of this group of crops are described.
References
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Journal ArticleDOI
Callus formation from stem protoplasts of corn (Zea mays L.)
TL;DR: The first successful culture, with sustained divisions, of protoplasts from intact plants of Zea mays is described, using a hanging microdrop array technique which permits the testing of very large numbers of different culture media and hormone variations.
In vitro genetics of cereals: problems and perspectives [maize, sorghum]
J.P. King,I. Potrykus,E. Thomas +2 more
Journal ArticleDOI
Differentiation in calli originated from isolated protoplasts of rice (Oryza sativa L.) through plating technique
Pradip C. Deka,S. K. Sen +1 more
TL;DR: Protoplasts from mesophyll cells and callus cells of rice (Oryza sativa L.) have been isolated by enzyme treatment and healthy root formation through transfer to suitable medium is up to now the morphogenetic reaction of the calli.
Journal ArticleDOI
Anatomy of Maize Tissue Cultures
R. L. Mott,W. W. Cure +1 more
TL;DR: Removal of exogenous growth regulators from the culture medium simply allowed outgrowth of existing root primordia as normal roots and this accounts for the characteristic ease of rhizogenesis from what has been called maize callus.
Journal ArticleDOI
Multiple-drop-array (MDA) technique for the large-scale testing of culture media variations in hanging microdrop cultures of single cell systems. I: The technique
I. Potrykus,C. T. Harms,H. Lörz +2 more
TL;DR: The MDA technique is suited for all single cell systems, including protoplasts, cells and microspores, and allows a small team to test 5000 media variations in 2- and 3-factor combinations with a single population of 2 × 10 6 cells within two days.
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