Journal ArticleDOI
Cancer chemoprevention by hydroxytyrosol isolated from virgin olive oil through G1 cell cycle arrest and apoptosis.
Roberto Fabiani,A. De Bartolomeo,Patrizia Rosignoli,Maurizio Servili,GianFrancesco Montedoro,Guido Morozzi +5 more
TLDR
The hypothesis that hydroxytyrosol may exert a protective activity against cancer by arresting the cell cycle and inducing apoptosis in tumour cells is supported and hydroxyTYrosol, an important component of virgin olive oil, may be responsible for its anticancer activity is suggested.Abstract:
Recent epidemiological evidence and animal studies suggest a relationship between the intake of olive oil and a reduced risk of several malignancies. The present study assesses the effect of hydroxytyrosol, a major antioxidant compound of virgin olive oil, on proliferation, apoptosis and cell cycle of tumour cells. Hydroxytyrosol inhibited proliferation of both human promyelocytic leukaemia cells HL60 and colon adenocarcinoma cells HT29 and HT29 clone 19A. The con-centrations of hydroxytyrosol which inhibited 50% of cell proliferation were approximately 50 and approximately 750 micromol/l for HL60 and both HT29 and HT29 clone 19A cells, respectively. At concentrations ranging from 50 to 100 micromol/l, hydroxytyrosol induced an appreciable apoptosis in HL60 cells after 24 h of incubation as evidenced by flow cytometry, fluorescence microscopy and internucleosomal DNA fragmentation. Interestingly, no effect on apoptosis was observed after similar treatment of freshly isolated human lymphocytes and polymorphonuclear cells. The DNA cell cycle analysis, quantified by flow cytometry, showed that the treatment of HL60 cells with hydroxytyrosol 50-100 micromol/l arrested the cells in the G0/G1 phase with a concomitant decrease in the cell percentage in the S and G2/M phases. These results support the hypothesis that hydroxytyrosol may exert a protective activity against cancer by arresting the cell cycle and inducing apoptosis in tumour cells, and suggest that hydroxytyrosol, an important component of virgin olive oil, may be responsible for its anticancer activity.read more
Citations
More filters
Journal ArticleDOI
Dietary Polyphenols and Their Biological Significance
TL;DR: The biological mechanisms of action and protective effects of dietary polyphenols are described, and the most recent literature on the subject is covered.
Journal ArticleDOI
Natural Phenolic Compounds From Medicinal Herbs and Dietary Plants: Potential Use for Cancer Prevention
TL;DR: This review covers the most recent literature to summarize structural categories and molecular anticancer mechanisms of phenolic compounds from medicinal herbs and dietary plants.
Journal ArticleDOI
Polyphenols and Human Health: Prevention of Disease and Mechanisms of Action
David Vauzour,Ana Rodriguez-Mateos,Giulia Corona,Maria Jose Oruna-Concha,Jeremy P. E. Spencer +4 more
TL;DR: The role that polyphenols play in the prevention of cancer, cardiovascular disease and neurodegeneration is provided and epidemiological data, human intervention study findings, as well as animal and in vitro studies in support of these actions are presented.
Journal ArticleDOI
Health and sensory properties of virgin olive oil hydrophilic phenols: agronomic and technological aspects of production that affect their occurrence in the oil.
Maurizio Servili,Roberto Selvaggini,Sonia Esposto,Agnese Taticchi,Gian Francesco Montedoro,Guido Morozzi +5 more
TL;DR: The sensory and healthy proprieties of VOO hydrophilic phenols as well as the agronomic and technological parameters that affect their concentration in the oil are discussed in this paper.
Journal ArticleDOI
Bioactivity and analysis of biophenols recovered from olive mill waste.
Hassan K. Obied,Malcolm S. Allen,Danny R. Bedgood,Paul D. Prenzler,Kevin Robards,Regine Stockmann +5 more
TL;DR: The phenolic profile of OMW is complex, yet this complexity has not been fully exploited in the valorization of the waste, and most work on the bioactivity of O MW has focused on antioxidant and antimicrobial activities.
References
More filters
Journal ArticleDOI
Principles of CDK regulation
TL;DR: The activity of cyclin-dependent kinases is controlled by four highly conserved biochemical mechanisms, forming a web of regulatory pathways unmatched in its elegance and intricacy.
Journal ArticleDOI
Requirement for Generation of H2O2 for Platelet-Derived Growth Factor Signal Transduction
TL;DR: The results suggest that H2O2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.
Journal ArticleDOI
Cyclins and cancer II: Cyclin D and CDK inhibitors come of age
Tony Hunter,Jonathon Pines +1 more
Journal ArticleDOI
Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure.
Manon van Engeland,Luc J. W. Nieland,Frans C. S. Ramaekers,Bert Schutte,Chris P. M. Reutelingsperger +4 more
TL;DR: The basic mechanisms underlying the loss of membrane asymmetry during apoptosis are described and the novel annexin V-binding assay is discussed, an extension to the current available methods.
Journal ArticleDOI
Simple and hydrolyzable phenolic compounds in virgin olive oil. 1. Their extraction, separation, and quantitative and semiquantitative evaluation by HPLC
TL;DR: In this article, the extractive and HPLC methods of analysis are examined and some modifications are presented by means of these techniques, elenolic acid and four unknown compounds having phenolic behavior were separated from virgin olive oil.