Patent
Carrier system and method for the introduction of genes into mammalian cells
George Y. Wu,Catherine H. Wu +1 more
Reads0
Chats0
TLDR
In this paper, a targetable gene delivery system is provided for introducing foreign genes into mammalian cells, which employs a soluble targetable DNA complex and utilizes receptor-mediated endocytosis to endow cell specificity.Abstract:
A targetable gene delivery system is provided for introducing foreign genes into mammalian cells. The system employs a soluble targetable DNA complex and utilizes receptor-mediated endocytosis to endow cell specificity. The soluble DNA-carrying complex is formed by non-covalently binding a ligand conjugate with the foreign gene. The conjugate, in turn, is formed by bonding receptor-specific ligands such as asialoglycoproteins to polycations such as polylysine through covalent bonds such as disulfide bonds. The system exhibits a high degree of cell specificity and offers potential for the treatment of inherited genetic disorders.read more
Citations
More filters
Patent
Composition for introducing nucleic acid complexes into higher eucaryotic cells
David T. Curiel,Max L. Birnstiel,Matthew Cotten,Ernst Wagner,Kurt Zatloukal,Christian Plank,Berndt Oberhauser,Walter G. M. Schmidt +7 more
TL;DR: A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acids and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated as mentioned in this paper.
Patent
Stable lipid-comprising drug delivery complexes and methods for their production
TL;DR: In this article, a stable, concentrated, biologically active and ready-to-use lipid-comprising drug delivery complexes and methods for their production are described and the biological activity of the complexes produced are comparable to the formulations prepared according to the prior art admixture method.
Patent
Packaging systems for human recombinant adenovirus to be used in gene therapy
TL;DR: In this article, an improved method and products based on adenoviral materials which can advantageously be used in for instance gene therapy are presented. But the method does not exclude the possibility of homologous recombination, thereby not allowing the formation of replication-compensated adnovirus and/or interference with the immune system.
Patent
Activation and expansion of cells
Ronald J. Berenson,Che Law,Mark Bonyhadi,Narinder Saund,Stewart Craig,Alan R. Hardwick,Dale Kalamasz,David Mcmillen,Harjinder Singh Chana +8 more
TL;DR: In this paper, a method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities is presented, where cells are activated and expanded to very high numbers of cells in a short period of time.
Patent
Conjugates for introducing nucleic acid into higher eucaryotic cells
TL;DR: In this paper, the authors describe conjugates in which a virus is bound via an antibody to a substance having an affinity for nucleic acid, for transporting gene constructs into higher eucaryotic cells.
References
More filters
Journal ArticleDOI
Receptor-mediated in vitro gene transformation by a soluble DNA carrier system.
George Y. Wu,C H Wu +1 more
TL;DR: There is evidence that foreign DNA can be specifically delivered to cells by a soluble carrier system that takes advantage of receptor-mediated endocytosis, and that competition by a 10-fold excess of ASOR prevented gene transformation by the ASOR X poly-L-lysine X DNA complex.
Journal ArticleDOI
American Association for the Study of Liver Diseases: Symposium on Toxic Hepatic Injury
Journal ArticleDOI
The galactose-specific recognition system of mammalian liver: The route of ligand internalization in rat hepatocytes
TL;DR: Serial sectioning indicated that tubular structures in Golgi-lysosome regions were often interconnected to the larger vesicles, but that tubules in the peripheral cytoplasm were only occasionally connected to larger structures.
Journal ArticleDOI
Evidence for targeted gene delivery to Hep G2 hepatoma cells in vitro.
George Y. Wu,Cathy H. Wu +1 more
TL;DR: A system for targeting foreign DNA to hepatocytes in vitro using a soluble DNA carrier that takes advantage of receptor-mediated endocytosis to achieve internalization is developed based on the fact that hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo)glycoproteins.
Journal ArticleDOI
Disulfide spacer between methotrexate and poly(D-lysine). A probe for exploring the reductive process in endocytosis.
TL;DR: It is concluded that the reductive process through which methotrexate is released from the disulfide spacer occurs inside cells and not at the cell surface, requires neither acid pH nor lysosomal enzymes, and is not mediated by a glutathione-disulfide exchange reaction requiring high glutathion concentrations.