Journal ArticleDOI
Cellulase immobilized on a soluble polymer
TLDR
The pH activity of immobilized enzyme was unchanged, but exhibited more stable activity at acidic pH than the free enzyme, and higher resistance to heat inactivation was also observed.Abstract:
Aspergillus niger cellulase was imobilized on cyanogen bromide activated dextran of varying molecular weights. The effect of different concentrations of cyanogen bromide used for the activation process was also studied. About 50% conjugation and 70% retention activity was achieved in the immobilized cellulase. The pH activity of immobilized enzyme was unchanged, but exhibited more stable activity at acidic pH than the free enzyme. Higher resistance to heat inactivation was also observed.read more
Citations
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Journal ArticleDOI
The kinetics and mechanism of shear inactivation of lipase from Candida cylindracea
Yuan-Kun Lee,Choon-Liang Choo +1 more
TL;DR: Temperature was found to have no effect in the rate of shear‐induced denaturation of the lipase in the range 20 to 30°C during shearing at 100 s −1and optimal pH, but above 30 °C, the rateof denaturationof the lipases increased drastically as a function of temperature.
Patent
Detergent compositions comprising immobilized enzymes
TL;DR: In this paper, the authors describe detergent compositions comprising one or more enzymes which are immobilized by a covalent binding on an activated polymer, such as polyethylene glycol.
Journal ArticleDOI
Removal of lignin and reuse of cellulases for continuous saccharification of lignocelluloses.
TL;DR: The ultra‐filtration membrane PM10 (Amicon) could be used for recovery of cellulases, but the membrane UH‐1 (Toyo Roshi) was better for this purpose, because no cellulases leaked from the membrane, and the amount of cellulase adsorbed to the membrane was less.
Journal ArticleDOI
Immobilized cellulases for cellulose utilization
TL;DR: The β-glucosidase component of cellulase can be stabilized and immobilized for efficient hydrolysis of soluble cellobiose and short-chain cello-oligosaccharides.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen Halides
TL;DR: Biologically active proteins and polypeptides can be coupled by various means to cellulose, starch and cross-linked polysaccharide gels such as ‘Sephadex’.