Journal ArticleDOI
Changes in axon fluorescence during activity: Molecular probes of membrane potential
Lawrence B. Cohen,Lawrence B. Cohen,Lawrence B. Cohen,Brian M. Salzberg,Brian M. Salzberg,Brian M. Salzberg,H. V. Davila,H. V. Davila,H. V. Davila,William N. Ross,William N. Ross,William N. Ross,D. Landowne,D. Landowne,D. Landowne,A. S. Waggoner,A. S. Waggoner,A. S. Waggoner,C. H. Wang,C. H. Wang,C. H. Wang +20 more
TLDR
The fluorescence of dyes added to squid giant axons was studied during action potentials and voltage-clamp steps to find large changes in fluorescence that would allow optical monitoring of membrane potential in neurons and other cells.Abstract:
The fluorescence of dyes added to squid giant axons was studied during action potentials and voltage-clamp steps. One goal was to find fluorescence changes related to the increases in membrane conductance that underlie propagation. A second goal was to find large changes in fluorescence that would allow optical monitoring of membrane potential in neurons and other cells. Attempts were made to measure fluorescence changes using over 300 different fluorescent molecules and positive results were obtained with more than half of these. No evidence was found that would relate, any of the fluorescence changes to the increases in membrane conductance that accompany depolarization; most, instead, were correlated with the changes in membrane potential. The fluorescence changes of several dyes were relatively large; the largest changes during an action potential were 10−3 of the resting intensity. They could be measured with a signal-to-noise ratio of better than 10∶1 in a single sweep.read more
Citations
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Fluorescent dyes and their supramolecular host/guest complexes with macrocycles in aqueous solution.
TL;DR: Fluorescent Dyes and Their Supramolecular Host/Guest Complexes with Macrocycles in Aqueous Solution and how these complexes interact with each other and with solvent-free substrates is studied.
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Voltage-sensitive dyes reveal a modular organization in monkey striate cortex.
Gary G. Blasdel,Guy Salama +1 more
TL;DR: In this paper, the same pattern of ocular dominance and orientation selectivity were obtained repeatedly from the same patch of cortex using the dye merocyanine oxazolone, together with current image processing techniques.
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Measurement of Intracellular Calcium
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Cortical point-spread function and long-range lateral interactions revealed by real-time optical imaging of macaque monkey primary visual cortex
TL;DR: The usefulness of real-time optical imaging in the study of population activity and the exploration of cortical dendritic processing is described, raising the possibility that distributed processing over a very large cortical area plays a major role in the processing of visual information by the primary visual cortex of the primate.
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VSDI: a new era in functional imaging of cortical dynamics
Amiram Grinvald,Rina Hildesheim +1 more
TL;DR: Imaging based on voltage-sensitive dyes (VSDI) offers the highest spatial and temporal resolution for imaging neocortical functions in the living brain, and has paved the way for a new era in the functional imaging of cortical dynamics.
References
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A. L. Hodgkin,A. F. Huxley +1 more
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Changes in absorption, fluorescence, dichroism, and birefringence in stained giant axons: Optical measurement of membrane potential
William N. Ross,William N. Ross,William N. Ross,Brian M. Salzberg,Brian M. Salzberg,Brian M. Salzberg,Lawrence B. Cohen,Lawrence B. Cohen,Lawrence B. Cohen,Amiram Grinvald,Amiram Grinvald,Amiram Grinvald,H. V. Davila,H. V. Davila,H. V. Davila,A. S. Waggoner,A. S. Waggoner,A. S. Waggoner,C. H. Wang,C. H. Wang,C. H. Wang +20 more