Journal ArticleDOI
Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus aurata).
TLDR
Zymograms of larval extracts indicated that exogenous food has more a qualitative than a quantitative role in the secretion of digestive enzymes in this species.Abstract:
The evolution of the digestive enzyme equipment in seabream from hatching to 30 days old larvae was studied; there was a progressive increase in the activity of protease, amylase and acid and alkaline phosphatase from day 15 onwards. The use of specific inhibitors, and SDS-PAGE provided evidence to suggest that most of the proteases belonged to the serine group. A high α-amylase activity was also denoted. Zymograms of larval extracts indicated that exogenous food has more a qualitative than a quantitative role in the secretion of digestive enzymes in this species.read more
Citations
More filters
Journal ArticleDOI
Digestive enzymes in fish larvae and juveniles—implications and applications to formulated diets
TL;DR: The results suggest that inclusion of bombesin may increase assimilation of diets and larval growth, however, other trials with juvenile fish have shown no effect of these additions of the neuropeptide.
Journal ArticleDOI
Feeding behaviour and digestive physiology in larval fish: current knowledge, and gaps and bottlenecks in research
TL;DR: A holistic understanding of feeding ecology and digestive functions is important for designing diets for fish larvae and the adaptation of rearing conditions to meet requirements for the best presentation of prey and microdiets, and their optimal ingestion, digestion and absorption.
Journal ArticleDOI
The onset of exogenous feeding in marine fish larvae
Manuel Yúfera,Maria J. Darias +1 more
TL;DR: This review will focus on the most relevant processes during this short period of early life history of marine fish: gut anatomy, digestive capacities, feeding behaviour and metabolism.
Journal ArticleDOI
Modifications of digestive enzymes in trout (Oncorhynchus mykiss) and sea bream (Sparus aurata) in response to dietary fish meal replacement by plant protein sources
Ester Santigosa,J. Sánchez,Françoise Médale,Sadasivam Kaushik,Jaume Pérez-Sánchez,M.A. Gallardo +5 more
TL;DR: Three experimental diets in which fish meal was partially or totally replaced by plant protein sources were fed to rainbow trout and gilthead sea bream to study the effects of these diets on digestive enzymes in comparison to fish fed fish meal diets (FM).
Journal ArticleDOI
Histological and histochemical development of the digestive system of Solea senegalensis (Kaup, 1858) larvae
TL;DR: Analysis of data obtained in this study suggests that after 31DAH S. senegalensis larvae are capable of ingesting, digesting and absorbing nutrients, having a morphologically complete digestive tract, equipped with digestive enzymes.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Crystalline soybean trypsin inhibitor : ii. general properties.
TL;DR: Crystalline soy protein when denatured is readily digestible by pepsin, and less readily by chymotrypsin and by trypsin, which results in a proportional gain in the inhibiting activity.
Journal ArticleDOI
Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors
TL;DR: A rapid, sensitive, and generally applicable substrate-sodium dodecyl sulfate-polyacrylamide gel electrophoresis method for detection of proteinases or proteinaceous protease inhibitors in biological preparations is described.