Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility
Mario Ollero,Enrique Gil-Guzman,Mari C. Lopez,Rakesh Sharma,Ashok Agarwal,K.L. Larson,Donald P. Evenson,Anthony J. Thomas,Juan G. Alvarez +8 more
TLDR
The high levels of ROS production and DNA damage observed in immature spermatozoa may be indicative of derangements in the regulation of spermiogenesis, which is implicated in the pathogenesis of male infertility.Abstract:
BACKGROUND: Reactive oxygen species (ROS)-induced damage of membrane phospholipids and DNA in human spermatozoa has been implicated in the pathogenesis of male infertility. In this study, variations in ROS production, DNA structure (as measured by the sperm chromatin structure assay) and lipid composition, were studied in human spermatozoa at different stages of maturation. METHODS: Sperm subsets were isolated by discontinuous density gradient centrifugation of semen samples obtained from healthy donors and from infertility patients. RESULTS: DNA damage and ROS production were highest in immature spermatozoa with cytoplasmic retention and abnormal head morphology, and lowest in mature spermatozoa. Docosahexaenoic acid and sterol content were highest in immature germ cells and immature spermatozoa, and lowest in mature spermatozoa. The relative proportion of ROS-producing immature spermatozoa in the sample was directly correlated with DNA damage in mature spermatozoa, and inversely correlated with the recovery of motile spermatozoa. There was no correlation between DNA damage and sperm morphology in mature spermatozoa. CONCLUSIONS: The high levels of ROS production and DNA damage observed in immature spermatozoa may be indicative of derangements in the regulation of spermiogenesis. DNA damage in mature spermatozoa may be the result of oxidative damage by ROS-producing immature spermatozoa during sperm migration from the seminiferous tubules to the epididymis.read more
Citations
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Journal ArticleDOI
Role of reactive oxygen species in the pathophysiology of human reproduction
TL;DR: High levels of ROS are detrimental to the fertility potential both in natural and assisted conception states.
Journal ArticleDOI
Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques.
Journal ArticleDOI
Role of sperm chromatin abnormalities and DNA damage in male infertility
Ashok Agarwal,Tamer M. Said +1 more
TL;DR: Screening for sperm DNA damage may provide useful information in cases of male idiopathic infertility and in those men pursuing assisted reproduction, and treatment should include methods for prevention of spermDNA damage.
Journal ArticleDOI
Polyunsaturated Fatty Acids in Male and Female Reproduction
TL;DR: In Westernized societies, average consumption of n-6 polyunsaturated fatty acids (PUFAs) far exceeds nutritional requirements, and adequate powered trials are required to determine the extent to which this aspect of the authors' diets does influence their fertility.
Journal ArticleDOI
Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis
TL;DR: The mechanisms responsible for DNA fragmentation in human sperm, including those occurring during spermatogenesis and transport through the reproductive tract are reviewed, and how abnormal sperm could be dealt with by the oocyte and how sperm DNA abnormalities may interfere with normal embryo and fetal development are discussed.
References
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World Health Organization Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 4th ed.
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Sperm morphologic features as a prognostic factor in in vitro fertilization.
Thinus F. Kruger,Roelof Menkveld,F. S. H. Stander,Carl Lombard,Jacobus P. Van der Merwe,Johannes A. van Zyl,Karen Smith +6 more
TL;DR: In this article, the authors conducted a prospective study in women with bilateral tubal damage to determine whether there is a prognostic value in the percentage normal sperm morphologic features in a human in vitro fertilization (IVF) program.
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Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic
Donald P. Evenson,Lorna K. Jost,D Marshall,M J Zinaman,E D Clegg,K Purvis,P. De Angelis,O P Claussen +7 more
TL;DR: Based on logistic regression, spermatozoa with denatured DNA (cells outside the main population, COMP alpha t) were the best predictor for whether a couple would not achieve pregnancy.
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Population study of causes, treatment, and outcome of infertility.
Michael G.R. Hull,C M Glazener,N J Kelly,David I. Conway,P A Foster,R. A. Hinton,C. Coulson,P. A. Lambert,E. M. Watt,K M Desai +9 more
TL;DR: Infertility was unexplained in 28% and the chance of pregnancy was mainly determined by duration of infertility, and in vitro fertilisation could benefit 80% of cases of tubal damage and 25% of unexplained infertility--that is, 18% of all cases, representing up to 216 new cases each year per million of the total population.
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Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity.
TL;DR: The results suggest that superoxide dismutase plays the major role in protecting human spermatozoa against lipid peroxidation, and the superoxide Dismutase activity of a fresh sperm sample appears to be a good predictor of the lifetime (up to the complete loss of motility) of that particular sample, and so may prove useful in semen analysis.