Chemical composition and bioactivity of essential oils and Ethanolic extracts of Ocimum basilicum L. and Thymus algeriensis Boiss. & Reut . from the Algerian Saharan Atlas
Maria Rezzoug,Boulanouar Bakchiche,Abdelaziz Gherib,Ascrizzi Roberta,FlaminiGuido,Özge Kilinçarslan,Ramazan Mammadov,Sanaa K. Bardaweel +7 more
TLDR
The results suggest that the bioactive compounds found in the ethanolic extracts and essential oils from Ocimum basilicum and Thymus algeriensis, with diverse antioxidant, antimicrobial and anticancer activities, may have beneficial applications in nutraceutical and pharmaceutical technologies.Abstract:
There is increasing interest in the pharmaceutical and food industries to substitute synthetic chemicals with naturally occurring compounds possessing bioactive properties. Plants are valuable sources of bioactive compounds. The present study investigates the chemical composition and antioxidant, antimicrobial, and anticancer activities of ethanolic extracts (EEs) and essential oils (EOs) from two species in the Lamiaceae family, Ocimum basilicum L. and Thymus algeriensis Boiss. & Reut., cultivated in the Algerian Saharan Atlas. The total flavonoid contents of the plants’ ethanolic extracts were determined by the aluminium chloride method, while the total phenols were determined using the Folin-Ciocalteu method. Essential oils were obtained by hydrodistillation of the aerial parts of the plants and were analysed by GC-MS. The free radical-scavenging ability and antioxidant potential of the plants’ EEs and EOs were probed using the 2, 2-diphenyl-picrylhydrazyl radical-scavenging, ABTS radical-scavenging, ferric-reducing power and phosphomolybdenum assays. The antimicrobial activities were evaluated against several pathogens characteristic of gram-negative bacteria (three species), gram-positive bacteria (three species) and fungi (two species). The microdilution method was used to estimate the minimum inhibitory concentrations (MICs). The oils’ anticancer potential against several cancer types was also studied using the MTT assay and reported as the toxic doses that resulted in a 50% reduction in cancer cell growth (LD50). Phenolic compounds in the EEs from both plants were analysed by HPLC and demonstrated a rich flavonoid content. Chemical analysis of the essential oil from Ocimum basilicum revealed 26 unique compounds, with linalool (52.1%) and linalyl acetate (19.1%) as the major compounds. A total of 29 compounds were identified in the essential oil from Thymus algeriensis, with α-terpinyl acetate (47.4%), neryl acetate (9.6%), and α-pinene (6.8%) as the major compounds. The ethanolic extracts and essential oils from both plants exhibited moderate antioxidant activities and moderate to weak antimicrobial activities. Furthermore, anticancer activities against the examined human cancer cell lines were associated with only the EOs from both plants, with LD50 values ranging between 300 and 1000 μg/mL. The results suggest that the bioactive compounds found in the ethanolic extracts and essential oils from Ocimum basilicum and Thymus algeriensis, with diverse antioxidant, antimicrobial and anticancer activities, may have beneficial applications in nutraceutical and pharmaceutical technologies.read more
Citations
More filters
Journal ArticleDOI
Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens.
TL;DR: The functional and molecular roles of medicinal plants and their bioactive compounds are considered, focusing typically on their antimicrobial activities against clinically important pathogens.
Journal ArticleDOI
Functional importance of bioactive compounds of foods with Potential Health Benefits: A review on recent trends
Kolawole Banwo,Ayoyinka Olufunke Olojede,A.T. Adesulu-Dahunsi,Deepak Kumar Verma,Mamta Thakur,Soubhagya Tripathy,Smita Singh,Smita Singh,Ami Patel,Alok Kumar Gupta,Cristóbal N. Aguilar,Gemilang Lara Utama +11 more
TL;DR: The current study considers the health potential of BACs and their rising demand in form of functional foods in the world and their analytical methods, bioavailability and bioaccessibility.
Journal ArticleDOI
Chemical Composition and Antimicrobial Activity of Essential Oils from the Aerial Parts of Pinus eldarica Grown in Northwestern Iran.
Tayyebeh Ghaffari,Hossein Samadi Kafil,Solmaz Asnaashari,Safar Farajnia,Abbas Delazar,Su Cheol Baek,Hamed Hamishehkar,Ki Hyun Kim +7 more
TL;DR: The antibacterial and antifungal properties of the EOs were examined: pollen EO exhibited antibacterial activity against Escherichia coli; bark EO inhibited the growth of Candida albicans and Staphylococcus aureus; and the needle EO inhibition the growth
Journal ArticleDOI
Perspectives on the Combined Effects of Ocimum basilicum and Trifolium pratense Extracts in Terms of Phytochemical Profile and Pharmacological Effects.
Andreea-Ina Antonescu Mintas,Florina Miere Groza,Luminita Fritea,Mariana Ganea,Mihaela Zdrinca,Luciana Dobjanschi,Angela Antonescu,Simona Ioana Vicas,Florin Bodog,Rakesh K. Sindhu,Simona Cavalu +10 more
TL;DR: In this paper, the therapeutic potential of Ocimum basilicum and Trifolium pratense in relation with their phytochemical profile and to highlight the pharmacological activity of aqueous or ethanol extracts is explored.
Journal ArticleDOI
Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells.
Khawlah Athamneh,Aysha Hamad Alneyadi,Halima Alsamri,Asma Alrashedi,Abdulrasheed Palakott,Khaled A. El-Tarabily,Ali H. Eid,Yusra Al Dhaheri,Rabah Iratni +8 more
TL;DR: OMEO inhibits colon cancer through p38 MAPK-mediated protective autophagy and apoptosis associated with caspase-dependent cleavage of p70S6K, a protein reported to be overexpressed in colon cancer and associated with drug resistance.
References
More filters
Journal ArticleDOI
Antioxidant activity applying an improved ABTS radical cation decolorization assay.
Roberta Re,Nicoletta Pellegrini,Anna R. Proteggente,Ananth S. Pannala,Min Yang,Catherine Rice-Evans +5 more
TL;DR: A method for the screening of antioxidant activity is reported as a decolorization assay applicable to both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants.
Book ChapterDOI
Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent
TL;DR: Aggregate analysis of this type is an important supplement to and often more informative than reems of data difficult to summarize from various techniques, such as high-performance liquid chromatography (HPLC) that separate a large number of individual compounds.
Journal ArticleDOI
Studies on products of browning reaction--antioxidative activities of products of browning reaction prepared from glucosamine
TL;DR: The BGA team discusses the development of G-15, which aims to address the challenge of “superbugs” in the high-acid environment.
Journal ArticleDOI
Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E.
TL;DR: The phosphomolybdenum method is routinely applied in the laboratory to evaluate the total antioxidant capacity of plant extracts and to determine vitamin E in a variety of grains and seeds, including corn and soybean.
Journal ArticleDOI
Identification of volatile components in basil Ocimum basilicum L and thyme leaves Thymus vulgaris L and their antioxidant properties
TL;DR: Aroma compounds in the extracts of basil leaves (Ocimum basilicum L.) and thyme leaves (Thymus vulgaris L.) were identified by gas chromatography and mass spectrometry (GC/MS).