Journal ArticleDOI
Choline: high-affinity uptake by rat brain synaptosomes.
TLDR
The high-affinity choline uptake appears to represent selective choline accumulation by cholinergic neurons, and is associated with considerable formation of acetylcholine.Abstract:
Synaptosomes from rat brain accumulate choline by two kinetically distinct processes, a high-affinity uptake system [Michaelis constant (K(m)) = 1 x 10(-6)M], and a low-affinity system (K(m) = 9 x 10(-5)M). The high-affinity uptake system requires sodium, and is associated with considerable formation of acetylcholine. The low-affinity uptake system is less dependent on sodium, and does not appear to be associated with a marked degree of acetylcholine formation. The high-affinity choline uptake appears to represent selective choline accumulation by cholinergic neurons.read more
Citations
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Journal ArticleDOI
Muscarinic Cholinergic Binding in Rat Brain
TL;DR: Binding sites with high affinity and specificity for [(3)H]quinuclidinyl benzilate (QNB) are present in homogenates of rat brain and the characteristics of the binding sites resemble those of muscarinic cholinergic receptors.
Journal ArticleDOI
High affinity transport of choline into synaptosomes of rat brain.
Henry I. Yamamura,S. H. Snyder +1 more
TL;DR: A variety of evidence suggests that the high affinity transport represents a selective accumulation of choline by cholinergic neurons, while the low affinity uptake system has some less specific function.
Journal ArticleDOI
Choline: selective accumulation by central cholinergic neurons.
TL;DR: The results suggest that when hippocampal synaptosomes are incubated in the presence of choline, at concentrations of 10 μm m or lower, then cholinergic nerve endings are responsible for the bulk of the choline accumulated by the tissue.
Journal ArticleDOI
Localization of transmitter candidates in the brain: the hippocampal formation as a model
Journal ArticleDOI
Sodium-dependent, high affinity choline uptake
Michael J. Kuhar,L C Murrin +1 more
TL;DR: This review will focus on choline transport into cholinergic neurons, especially by means of the sodium-dependent, high affinity choline uptake system (SDHACU) which appears to be involved in ACh synthesis and its regulation.
References
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High affinity transport of choline into synaptosomes of rat brain.
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