Journal ArticleDOI
Comparison of mechanisms of interaction between protein A from Staphylococcus aureus and human monoclonal IgG, IgA and IgM in relation to the classical FC gamma and the alternative F(ab')2 epsilon protein A interactions
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TLDR
The results indicate the existence of a common and variably expressed protein A reactivity in at least four of five human immunoglobulins, and suggest that an interaction with protein A cannot be used as a criterion for subclass differentiation of IgA and IgM.Abstract:
Four purified human monoclonal IgG, IgA and IgM proteins were tested for their inhibitory effect on the binding of protein-A-reactive 125I-IgE and 125I-Fc gamma, respectively, to protein-A-Sepharose. Only IgG myeloma proteins significantly inhibited the binding of 125I-Fc gamma to protein-A-Sepharose, whereas most, but not all, myeloma proteins, irrespective of their immunoglobulin class and with varying efficiency, inhibited the binding of protein-A-reactive 125I-IgE to protein-A-Sepharose. The inhibitory effect of IgG and IgA proteins on the binding of protein-A-reactive 125I-IgE was retained in the respective F(ab')2 fragments, whereas the inhibitory effect of IgG proteins on the binding of 125I-Fc gamma to protein-A-Sepharose was exclusively expressed in the Fc gamma fragment. In addition to the classical Fc gamma-protein A interaction, the results indicate the existence of a common and variably expressed protein A reactivity in at least four of five human immunoglobulins. The data suggest that an interaction with protein A cannot be used as a criterion for subclass differentiation of IgA and IgM.read more
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Book ChapterDOI
Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci
TL;DR: This chapter discusses the occurrence, properties, applications, biological activity, and potential significance of Ig receptors produced mainly by staphylococci and streptococci, more broadly classified more broadly as Ig receptors.
Journal ArticleDOI
Binding of immunoglobulins to protein A and immunoglobulin levels in mammalian sera.
TL;DR: The use of protein A from S. aureus as an anti-IgG reagent in immunological techniques has extended in recent years, together with knowledge about its interaction with immunoglobulins of different species.
Journal ArticleDOI
Future of antibody purification.
TL;DR: A wide survey of technologies that are competing to be part of a platform, and an assessment of those that have the most promise are provided.
Journal ArticleDOI
All individual domains of staphylococcal protein A show Fab binding
TL;DR: The interactions between the individual domains of staphylococcal protein A and Fc and Fab regions of human immunoglobulins were studied using real-time biospecific interaction analysis and for the first time, binding to human Fab was demonstrated for all native SPA domains.
Journal ArticleDOI
Subversion of the immune system by pathogens
Philippa Marrack,John W. Kappler +1 more
References
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Journal Article
"Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin.
Arne Forsgren,John Sjöquist +1 more
TL;DR: Protein A was isolated from S. aureus and shown to precipitate about 45% of a pooled normal human γG preparation, but this reaction is not a true antigen-antibody reaction.
Journal ArticleDOI
Use of staphylococcal protein A as an immunological reagent
TL;DR: Protein A has proven useful for the study of antigens and receptors on the surface of intact cells, and for the detection of antibody-secreting cells, so the use of protein A is now the method of choice for many preparative and analytical purposes in immunology.
Journal Article
Differences in anti-protein A activity among IgG subgroups.
Göran Kronvall,Ralph C. Williams +1 more
TL;DR: It appears that anti-protein A reactivity is confined to γG-1, γ G-2 and γE4 molecules, and the presence of protein A-reactive sites is confirmed.
Journal Article
Phylogenetic insight into evolution of mammalian Fc fragment of gamma G globulin using staphylococcal protein A.
TL;DR: Protein A may provide a useful tool in the study of the evolution of γG globulin and a comparison of patterns of reactivity between protein A and isolated human rheumatoid factors showed that in the majority of instances different specificities were involved.
Journal ArticleDOI
Recognition of Two Distinct Groups of Human IgM and IgA Based on Different Binding to Staphylococci
Morten Harboe,I. Følling +1 more
TL;DR: Polyclonal IgM from all of 17 individuals tested inhibited the binding of IgM Se to staphylococci, which indicates that the distinction corresponds to an IgM subclass rather than to an allotype.
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"Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin.
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