Journal ArticleDOI
Compositional analysis of small peptides using capillary electrophoresis and Ru(bpy)33+-based chemiluminescence detection
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TLDR
Ru(bpy)33+-based chemiluminescence detection of underivatized amino acids was coupled to capillary electrophoresis and evaluated as a separation and detection methodology for the compositional analysis of peptides and proteins.About:
This article is published in Microchemical Journal.The article was published on 2000-09-04. It has received 38 citations till now. The article focuses on the topics: Capillary electrophoresis & Amino acid.read more
Citations
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Journal ArticleDOI
Tris(2,2´-bipyridyl)ruthenium(II) chemiluminescence
TL;DR: This paper critically reviews analytical applications of the chemiluminescence from tris(2,2'-bipyridyl)ruthenium(II) and related compounds published in the open literature between mid-1998 and October 2005.
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Coreactants of tris(2,2′-bipyridyl)ruthenium(II) Electrogenerated Chemiluminescence
TL;DR: In this article, the authors comprehensively review typical coreactants, such as oxalate, persulfate, tripropylamine (TPA), NADH (Nicotinamide adenine dinucleotide, reduced disodium salt), 2-(dibutylamino)ethanol, vitamin C derivatives, alcohols, ketones, nucleic acid, formic acid and formaldehyde, organic acids, amines, hydrogen peroxide, oxygen, hydrazine and relative derivatives.
Journal ArticleDOI
Electrogenerated chemiluminescence detection of amino acids based on precolumn derivatization coupled with capillary electrophoresis separation.
TL;DR: A novel method for highly sensitive detection of primary and secondary amino acids with selective derivatization using acetaldehyde as a new derivatized reagent was proposed by capillary electrophoresis and electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(II).
Journal ArticleDOI
New technique for capillary electrophoresis directly coupled with end-column electrochemiluminescence detection.
TL;DR: A new end‐column electrochemiluminescence (ECL) detection technique coupling to capillary electrophoresis (CE) is characterized and lidocaine spiked in a urine sample was determined.
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Determination of diphenhydramine by capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection.
TL;DR: Tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection in a capillary electrophoresis separation system was used for the determination of diphenhydramine and gave satisfactory precision.
References
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Journal ArticleDOI
Rapid analysis of amino acids using pre-column derivatization.
TL;DR: The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydrolyzates and the liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids.
Journal ArticleDOI
Role of electron-donating/withdrawing character, pH, and stoichiometry on the chemiluminescent reaction of tris(2,2'-bipyridyl)ruthenium(III) with amino acids
TL;DR: In this paper, a postcolumn chemiluminescent technique for the detection of underivatized amino acids using electrogenerated tris(2,2'-bipyridyl)ruthenium(III) is described.
Journal ArticleDOI
Capillary electrophoresis with electrochemical detection employing an on-column Nafion joint
Thomas J. O'Shea,Robin D. Greenhagen,Susan M. Lunte,Craig E. Lunte,Malcolm R. Smyth,Donna M. Radzik,Nori Watanabe +6 more
TL;DR: In this article, an on-column joint utilizing Nafion tubing for the isolation of the electrical circuit from the detection end of a capillary zone electrophoresis system is described.
Journal ArticleDOI
Chemiluminescence Detection of Amino Acids, Peptides, and Proteins Using Tris-2,2′-Bipyridine Ruthenium (III)
TL;DR: In this paper, the feasibility of using the tris-2-2′-bipyridine ruthenium (III) (Ru(bpy)3 3 +) chemiluminescent (CL) reaction for the detection of amino acids, peptides, and proteins has been studied.
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In situ cell for electrochemically generated Ru(bpy)33+-based chemiluminescence detection in capillary electrophoresis
Xin Wang,Donald R. Bobbitt +1 more
TL;DR: In this article, a continuous in situ generation scheme was proposed to detect most amines and amino acids without derivatization, thus facilitating the optimization of the CE separation process independent of the detection method.