Computational adaptive optics for live three-dimensional biological imaging.
TLDR
Light microscopy of thick biological samples, such as tissues, is often limited by aberrations caused by refractive index variations within the sample itself, so this approach will open possibilities to study weakly labeled molecules in difficult-to-image live specimens.Abstract:
Light microscopy of thick biological samples, such as tissues, is often limited by aberrations caused by refractive index variations within the sample itself. This problem is particularly severe for live imaging, a field of great current excitement due to the development of inherently fluorescent proteins. We describe a method of removing such aberrations computationally by mapping the refractive index of the sample using differential interference contrast microscopy, modeling the aberrations by ray tracing through this index map, and using space-variant deconvolution to remove aberrations. This approach will open possibilities to study weakly labeled molecules in difficult-to-image live specimens.read more
Citations
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A guide to super-resolution fluorescence microscopy
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Tomographic phase microscopy
TL;DR: In this article, the authors present a system and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle.
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Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues
TL;DR: An approach to adaptive optics in microscopy wherein the rear pupil of an objective lens is segmented into subregions, and light is directed individually to each subregion to measure, by image shift, the deflection faced by each group of rays as they emerge from the objective and travel through the specimen toward the focus.
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Adaptive aberration correction in a confocal microscope
TL;DR: An adaptive confocal fluorescence microscope incorporating this modal sensor together with a deformable membrane mirror for aberration correction is demonstrated, which shows considerable improvement in contrast and apparent restoration of axial resolution.
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Adaptive optics in microscopy
TL;DR: In this article, the sources of aberrations, their effects and their correction with adaptive optics, particularly in confocal and two-photon microscopes, are discussed. And applications of adaptive optics in the related areas of optical data storage, optical tweezers and micro/nanofabrication are also reviewed.
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