Journal ArticleDOI
Corchorus olitorius (jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77).
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TLDR
In both 48 h leaf and seed extract treatments, genotoxic damage significantly increased with increasing concentrations at relevant cytotoxic concentrations, and the results of the comet assay revealed that the extracts induced genot toxic damage on ARH-77 cells.Abstract:
Context: Corchorus olitorius L. (Malvaceae) has industrial importance in world jute production and is a widely cultivated and consumed crop in Cyprus and in some Arabic countries.Objective: The present study investigated cytotoxic and genotoxic effects of leaf extracts (LE) and seed extracts (SE) of the C. olitorius on the multiple myeloma-derived ARH-77 cells. The extracts were also evaluated for their total phenol content (TPC) and free radical scavenging activity (FRSA).Materials and methods: C. olitorius was collected from Nicosia, Cyprus. TPC and FRSA were measured by Folin–Ciocalteu and DPPH free radical methods, respectively. Cytotoxicity was evaluated by the MTT assay (4–2048 µg/mL range), and DNA damage (at IC50 and ½IC50) was measured by the comet assay.Results and discussion: The LE had significantly higher total phenol (78 mg GAE/g extract) than the SE (2 mg GAE/g extract) with significantly higher FRSA (IC50 LE: 23 µg/mL and IC50 SE: 10 401 µg/mL). Both LE and SE exerted cytotoxic eff...read more
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Book ChapterDOI
Anticancer Activities of African Medicinal Spices and Vegetables
TL;DR: The likely mode of action of reported extracts and compounds included induction of apoptosis, coupled to cell cycle arrest either in G0/G1 or between G0-G1 and S-phases in cancer cells, disruption of the mitochondrial membrane potential, generation of reactive oxygen species as well as activation of caspases enzymes.
Journal ArticleDOI
African Green Leafy Vegetables Health Benefits Beyond Nutrition
TL;DR: This review aims at providing information that promotes increased utilization and consumption of ALVs as not only a source of nutrients but antioxidants and other beneficial bioactive components using cellular models.
Journal ArticleDOI
Health-promoting properties of Corchorus leaves: A review
TL;DR: The study revealed that the genus Corchorus is rich in bioactive molecules such as glycosides, polysaccharides, triterpenes, ionones, phenolics, sterols, and fatty acids that impart a range of prophylactic and therapeutic applications to the genus.
Journal ArticleDOI
Liposomal drug delivery of Corchorus olitorius leaf extract containing phytol using design of experiment (DoE): In-vitro anticancer and in-vivo anti-inflammatory studies.
Mohammad H. Shariare,Humaira Binte Noor,Junayet Hossain Khan,Jamal Uddin,Syed Rizwan Ahamad,Mohammad A. Altamimi,Fars K. Alanazi,Mohsin Kazi +7 more
TL;DR: In this paper, the authors developed liposomal drug delivery of Corchorus olitorius leaf extract with an average particle size below 150 nm and drug loading efficiency of ≥ 85 %.
Journal ArticleDOI
Anticancer Effects of the Corchorus olitorius Aqueous Extract and Its Bioactive Compounds on Human Cancer Cell Lines
John Paul Sese Tosoc,John Paul Sese Tosoc,Olga M. Nuñeza,Thangirala Sudha,Noureldien H. E. Darwish,Noureldien H. E. Darwish,Shaker A. Mousa +6 more
TL;DR: In this paper, the authors evaluated the anticancer properties, such as the antiproliferative, anti-angiogenic, and antitumor activities, of the C. olitorius aqueous extract (CO) and its bioactive compounds, chlorogenic acid (CGA) and isoquercetin (IQ), against human melanoma (A-375), gastric cancer (AGS), and pancreatic cancer (SUIT-2), using in vitro and in ovo biological assays.
References
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Journal Article
Total Phenol Analysis: Automation and Comparison with Manual Methods
TL;DR: In this article, a 40-sample/hour procedure was adapted from the Singleton-Rossi method of analysis for total phenols in wine and other plant extracts, and compared with small-volume manual and semi-automated versions of this analysis.
Journal ArticleDOI
Natural Products as Sources of New Drugs over the Period 1981−2002
TL;DR: From the data presented, the utility of natural products as sources of novel structures, but not necessarily the final drug entity, is still alive and well, and in the area of cancer, the percentage of small molecule, new chemical entities that are nonsynthetic has remained at 62% averaged over the whole time frame.
Journal ArticleDOI
On tyrosine and tryptophane determinations in proteins
Otto Folin,Vintila Ciocalteu +1 more
TL;DR: In this article, the authors summarized research into the existing methods for the quantitative determination of tyrosine and tryptophane in proteins, including the Folin-Looney method, which is based on reaction of a phosphotungstic phosphomolybdic acid in a phenol solution.
Journal ArticleDOI
DPPH antioxidant assay revisited
Om P. Sharma,T.K. Bhat +1 more
TL;DR: In this paper, the authors present a perspective of the protocols followed by different workers with incongruity in their results and recommend a standard procedure within the sensitivity range of spectrophotometry.