Journal ArticleDOI
Corrinoids from Methanosarcina barkeri: Structure of the α-ligand
TLDR
In this paper, 5-hydroxybenzimidazolylcobamide is the only base detected in cobamide compounds from methanol-grown Methanosarcina barkeri, accounting for about 83 and 90% of the total corrinoids of whole cells and cell-free extracts, respectively.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1982-09-30. It has received 77 citations till now. The article focuses on the topics: Corrinoids & Methanosarcina barkeri.read more
Citations
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Journal ArticleDOI
Methanogens and the diversity of archaebacteria.
Journal ArticleDOI
Vitamin B12 as a modulator of gut microbial ecology.
TL;DR: Although cobalamin is synthesized by some human gut microbes, it is a precious resource in the gut and is likely not provisioned to the host in significant quantities, however, this vitamin may make an unrecognized contribution in shaping the structure and function of human gut microbial communities.
Journal ArticleDOI
The bioenergetics of methanogenesis.
TL;DR: The reduction of CO2 or any other methanogenic substrate to methane serves the same function as the reduction of oxygen, nitrate or sulfate to more reduced products and these exergonic reactions are coupled to the production of usable energy generated through a charge separation and a protonmotive-force-driven ATPase.
Journal ArticleDOI
Isolation of an enzyme complex with carbon monoxide dehydrogenase activity containing corrinoid and nickel from acetate-grown Methanosarcina thermophila.
TL;DR: Fast protein liquid chromatography of cell extract from methanol- or acetate-grown Methanosarcina thermophila resolved two peaks of CO dehydrogenase activity, which imply an involvement in the dissimilation of acetate to methane, possibly by cleavage of acetates or activated acetate.
Journal ArticleDOI
Methyltransferases involved in methanol conversion by Methanosarcina barkeri.
Peter van der Meijden,Henk J. Heythuysen,Aloys Pouwels,Frans Houwen,Chris van der Drift,Godfried D. Vogels +5 more
TL;DR: The enzyme system involved in the methyltransfer from methanol to 2-mercaptoethanesulfonate (HS-CoM) was resolved into two enzyme fractions and one enzyme appears to be a cobalamin-containing protein, which is oxygen sensitive.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
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Methanobacillus omelianskii, a Symbiotic Association of Two Species of Bacteria*
TL;DR: The results indicate that M. omelianskii maintained in ethanol media is actually a symbiotic association of the two species.
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Biosynthesis of dimethylarsine by Methanobacterium.
B. C. McBride,R. S. Wolfe +1 more
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Proposed structure for coenzyme F420 from Methanobacterium.
L D Eirich,G D Vogels,R S Wolfe +2 more
TL;DR: Results of analysis of hydrolytic fragments and periodate oxidation products of the coenzyme, by infrared, UV-visible, 1H and 13CNMR spectrometry, mass spectromaetry, and quantitative elemental analyses indicate that coen enzyme F420 is N-[N-[O-[5-(8-hydroxy-5-deazaisoalloxazin-10-yl)-2,3,4-triHydroxy-4-pentoxyhydroxyphosphinyl