Showing papers in "Biochemical and Biophysical Research Communications in 1982"

TL;DR: The results suggest that R • induced damage may provide a stimulus to the mitochondrial biogenesis which results from endurance training.

TL;DR: It is apparent that phenyl-SepHarose offers several advantages over phenothiazine-Sepharose for affinity purification of calmodulin, and the time required for this procedure is substantially less than that of previously described procedures.

TL;DR: Results show that vanadate is a potent and selective inhibitor of phosphotyrosyl-protein phosphatase.

TL;DR: Electric impulses in the intensity range of 5–10 kV/cm with a duration of 5-10 μs were found to appreciably increase the uptake of DNA into cells, which appears unique in its ease and simplicity.

TL;DR: Platelet-derived arachidonate may serve as precursor for the neutrophil-derived eicosanoids LTB 4 and 5-HETE, and it is suggested that platelet- derived 12- HETE can be converted to DHETE by human neutrophils.

TL;DR: Hydrophobic high-performance liquid chromatography of reduced and alkylated 125I-labeled platelet-derived growth factor resulted in the separation of two distinct radioactive components, leading to inhomogeneity in the intact PDGF molecule.

TL;DR: Baicalein selectively inhibited platelet lipoxygenase, but it was less potent (ID 50 =100 μM), and other flavonoids tested had no inhibitory effect on either enzyme.

TL;DR: Kinetic studies showed that LTB4 activates PMN with respect to aggregation, mobilization of membrane-associated Ca2+, −˙ generation, and degranulation within seconds of exposure and indicate that L TB4 serves as a complete secretagogue.

TL;DR: The dynamic and opposite roles exerted by somatocrinin and somatostatin in regulating GH secretion are demonstrated in conscious freely-moving rats.

TL;DR: A new approach for the reaction of Sepharose with cyanogen bromide is described, using triethylamine as a “cyano-transfer” reagent, and an optimized procedure for activation at neutral pH was developed.

TL;DR: The findings indicate that polymyxin B, a surface active agent, effectively inhibited the phospholipid-sensitive enzyme presumably by interacting with phosphatidylserine.

TL;DR: Latent collagenase was activated by brief exposure to SDS and resolved by acrylamide slab gel electrophoresis in the presence of this detergent, suggesting a precursor-product relationship between the two sets of double bands.

TL;DR: When labeled 1-aminocyclopropane-1-carboxylic acid, an ethylene precursor, was administered to light-grown wheat leaves, it was primarily converted into a nonvolatile metabolite, which was identified as 1-(malonylamino)cyclopropane the conjugate in the wilted wheat leaves.

TL;DR: The inhibition of [3H]nitrendipine binding by verapamil was not of the simple competitive type but resembled allosteric inhibition, suggesting that there are at least two binding sites for calcium antagonists.

TL;DR: The peptide (chicken LH-RH) stimulates secretion of both LH and FSH from rat anterior pituitary cells and one possible structural candidate for this peptide is [Gln 8 ]-LH-RH, which differs from mammalian LH- RH only in that one Arg residue is replaced by a Glx residue.

TL;DR: The lectin could be extracted from Streptococcus sanguis with lithium 3,5-diiodosalicylate with specificity towards the major acidic oligosaccharide of human salivary mucin and incubation of LIS extracts with carbohydrate ligands demonstrated that the specificity of binding was NeuAcα2,3Galβ1,3[ 3 H-]GalNAcol ⪢ Gal β1, 3[ 3H-] GalNAcol .

TL;DR: A potent anticoagulant, named tentatively anti-LPS factor, which inhibits the endotoxin-mediated coagulation reaction, was found in both amebocytes from the hemolymphs of Tachypleus tridentatus and Limulus polyphemus.

TL;DR: In an in vivo comparison of 2′NDG with five other known antiherpetic agents including acyclovir for protection of mice against HSV-1 induced encephalitis, 2′ NDG was at least 60-fold more effective.

TL;DR: Results suggest that in contrast to cultured skin fibroblasts, liver may be able to introduce into lysosomes acid hydrolases that lack the mannose 6-phosphate recognition marker.

TL;DR: In this paper, the conditions of moderate heat pretreatment required to induce thermotolerance were established for Saccharomyces cerevisiae and they identified ethanol as an inducer of heat shock proteins.

TL;DR: The results indicate the acetyltransferase responsible for 1-alkyl-2-acetyl- sn -glycero-3-phosphocholine synthesis may serve an important role in human blood cells that release this biologically active phospholipid.

TL;DR: GM 3 or GM 1 ganglioside exogenously added in chemically-defined medium incorporate equally into cells, however, only GM 3 showed a significant growth inhibition to hamster fibroblasts, suggesting that GM 3 may regulate the function of the receptors for growth hormones.

TL;DR: The results demonstrated that prostaglandin D2, acting on the preoptic area, may play an important role in inducing sleep as a chemical mediator.

TL;DR: High affinity binding of the Ca 2+ -channel antagonist nitrendipine has been measured in microsomal membrane fractions from guinea pig ileal longitudinal smooth muscle and was sensitive to displacement by a series of 1,4-dihydropyridine analogs of nifedipine.

TL;DR: Cultures of rabbit fibroblasts with mouse tumor cells of epithelial origin showed increased collagenase production when compared to cultures of the individual cells, dependent on the cellular ratio between the normal fibro Blasts and tumor cells and was mediated by a soluble factor produced by the tumor cells.

TL;DR: It is suggested that LTB 4 may play an important role in immuno-regulation during hypersensitivity reactions and is also a potent inducer of suppressor cell activity at concentrations as low as 1 × 10 −14 M.

TL;DR: Through use of hydrophobic fluorescent probes, verapamil, and another calmodulin antagonist, proadifen, were shown to interact directly withCalmodulin in a manner that differed from the interaction of calmodul with trifluoperazine.

TL;DR: The results suggest that there may be multiple binding sites for different “subclasses” of calcium channel inhibitors, and that drug binding sites may be different molecular entities from the putative calcium channels.

TL;DR: PGE2 releases GH with a dose-response curve different from that of GRF, and the combination of PGE2 + GRF produces an Emax far greater than that due to either agonist alone; showing a true additivity.

TL;DR: Interphotoreceptor matrix material from bovine eyes contains 1.8 nmoles per eye of vitamin A in light- Adapted eyes and 0.7 in dark-adapted eyes, nearly all as unesterified retinol, which constitutes a significant fraction of theitamin A in the retina-IPM-retinal pigment epithelium functional complex.