Journal ArticleDOI
Detection and molecular characterization of bovine leukemia virus in Philippine cattle
Meripet Polat,Ayumu Ohno,Shin-nosuke Takeshima,Jiyun Kim,Mari Kikuya,Yuki Matsumoto,Claro N. Mingala,Misao Onuma,Yoko Aida +8 more
TLDR
This study provides comprehensive information regarding BLV infection levels in the Philippines and documents the presence of two BLV genotypes, genotypes 1 and 6, in this population.Abstract:
Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. However, there are no comprehensive studies on the distribution of BLV in the Philippines, and the genetic characteristics of Philippine BLV strains are unknown. Therefore, the aim of this study was to detect BLV infections in the Philippines and determined their genetic variability. Blood samples were obtained from 1116 cattle from different farms on five Philippine islands, and BLV provirus was detected by BLV-CoCoMo-qPCR-2 and nested PCR targeting BLV long terminal repeats. Out of 1116 samples, 108 (9.7 %) and 54 (4.8 %) were positive for BLV provirus, as determined by BLV-CoCoMo-qPCR-2 and nested PCR, respectively. Of the five islands, Luzon Island showed the highest prevalence of BLV infection (23.1 %). Partial env gp51 genes from 43 samples, which were positive for BLV provirus by both methods, were sequenced for phylogenetic analysis. Phylogenetic analysis based on a 423-bp fragment of the env gene revealed that Philippine BLV strains clustered into either genotype 1 or genotype 6. Substitutions were mainly found in antigenic determinants, such as the CD4+ T-cell epitope, the CD8+ T-cell epitope, the second neutralizing domain, B and E epitopes, and these substitutions varied according to genotype. This study provides comprehensive information regarding BLV infection levels in the Philippines and documents the presence of two BLV genotypes, genotypes 1 and 6, in this population.read more
Citations
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Journal ArticleDOI
Epidemiology and genetic diversity of bovine leukemia virus
TL;DR: This review provides a summary of available information about commonly used diagnostic approaches for the detection of BLV infection, including both serological and viral genome-based methods.
Journal ArticleDOI
A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis
Meripet Polat,Shin-nosuke Takeshima,Kazuyoshi Hosomichi,Jiyun Kim Kim,T. Miyasaka,Kazunori D. Yamada,Mariluz Arainga,Tomoyuki Murakami,Yuki Matsumoto,Veronica de la Barra Diaz,Carlos Javier Panei,Ester Teresa González,Misao Kanemaki,Misao Onuma,Guillermo Giovambattista,Yoko Aida +15 more
TL;DR: It is concluded that at least seven BLV genotypes (genotypes-1, -2, -4, -5, -6, -7, and -9) are circulating in South America, and a new genotype, genotype-9, in Bolivia is discovered.
Journal ArticleDOI
Risk factors associated with increased bovine leukemia virus proviral load in infected cattle in Japan from 2012 to 2014
TL;DR: The results showed that the proviral load increased with progression of lymphocytosis, and proviral loads were higher in contact breeding systems than in non-contact breeding systems, and higher in multiparous cattle than in nulliparous cows.
Journal ArticleDOI
Molecular epidemiological and serological studies of bovine leukemia virus (BLV) infection in Thailand cattle
EunJung Lee,Eunju Kim,Jadsada Ratthanophart,Ratchaneekorn Vitoonpong,Bo-Hye Kim,In-Soo Cho,Jae-Young Song,Kyoung-Ki Lee,Yeun-Kyung Shin +8 more
TL;DR: It is suggested that intensive surveillance and effective prevention strategies are required to determine the prevalence of BLV in Thailand and control continuous infections with BLVs.
Journal ArticleDOI
The molecular epidemiological study of bovine leukemia virus infection in Myanmar cattle.
TL;DR: Information is provided regarding BLV infection levels in Myanmar and confirms that genotype-10 is circulating in Myanmar, which is the most common neoplastic disease of cattle worldwide.
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