Journal ArticleDOI
Determination of carbonyl groups in oxidatively modified proteins by reduction with tritiated sodium borohydride
TLDR
A method to quantify oxidatively modified proteins through reduction of these carbonyl groups with tritiated borohydride is described, which provided excellent sensitivity and was directly proportional to the amount of protein.About:
This article is published in Analytical Biochemistry.The article was published on 1989-03-01. It has received 280 citations till now. The article focuses on the topics: Borohydride & Amino acid.read more
Citations
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Book ChapterDOI
Determination of carbonyl content in oxidatively modified proteins.
Rodney L. Levine,Donita Garland,Cynthia N. Oliver,Adolfo Amici,Isabel Climent,Anke-G. Lenz,Bong-Whan Ahn,Shmuel Shaltiel,Earl R. Stadtman +8 more
TL;DR: This chapter discusses methods to determine carbonyl content in oxidatively modified proteins and quantitated protein-bound pyruvoyl groups through formation of a Schiff base with p-aminobenzoic acid followed by reduction with cyanoborohydride.
Journal ArticleDOI
Protein carbonyl groups as biomarkers of oxidative stress.
TL;DR: The usage of protein CO groups as biomarkers of oxidative stress has some advantages in comparison with the measurement of other oxidation products because of the relative early formation and the relative stability of carbonylated proteins.
Journal ArticleDOI
Free radical-mediated oxidation of free amino acids and amino acid residues in proteins.
TL;DR: It is evident that the cyclic oxidation and reduction of the sulfur-containing amino acids may serve as an important antioxidant mechanism, and also that these reversible oxidations may provide an important mechanism for the regulation of some enzyme functions.
Journal ArticleDOI
Metal ion-catalyzed oxidation of proteins: Biochemical mechanism and biological consequences
TL;DR: The demonstration shows that oxidative modification of proteins "marks" them for degradation by most common proteases and especially by the cytosolic multicatalytic proteinase from mammalian cells, and contributes substantially to the intracellular pool of catalytically inactive and less active, thermolabile forms of enzymes.
Journal ArticleDOI
Free radicals, reactive oxygen species, oxidative stress and its classification
TL;DR: ROS homeostasis is described, principles of their investigation and technical approaches to investigate ROS-related processes are described, and a classification of oxidative stress based on its intensity is proposed.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Biology of disease: free radicals and tissue injury.
Bruce A. Freeman,James D. Crapo +1 more
TL;DR: This review surveys cellular sources of free radicals and the reactions they can undergo and discusses cellular defenses and adaptive mechanisms.
Journal ArticleDOI
Age-related changes in oxidized proteins.
TL;DR: The results suggest that loss of functional enzyme activity and increased heat lability of enzymes during aging may be due in part to oxidative modification by mixed function oxidation systems.