Journal ArticleDOI
Determination of phospholipids in vegetable oil by fourier transform infrared spectroscopy
J. M. Nzai,A. Proctor +1 more
TLDR
In this article, a method was developed to determine the total phospholipid content in vegetable oil by Fourier transform infrared spectroscopy (FTIR), which was found to be a useful analytical tool for simple and rapid quantitative determination.Abstract:
A method was developed to determine the total phospholipid content in vegetable oil by Fourier transform infrared spectroscopy (FTIR). Calibration curves of I-α-phosphatidylcholine (PC), I-α-phosphatidylethanolamine (PE), and I-α-phosphatidylinositol (PI) in hexane were generated at different concentrations. The optimal phospholipid absorption bands between 1200–970 cm−1 were identified and used for quantitative determination. High R
2≥0.968 were observed between band areas and phospholipid standard concentrations. Phospholipids from crude soybean oil were obtained by water degumming, and purification was performed on a silicic acid column. The phospholipid contents of purified phospholipid extract, degummed and crude soybean oil determined from calibration equations were >90, 0.0113, and 1.77%, respectively. High correlations of determination (R
2≥0.933) were observed between the FTIR method and thin-layer chromatography-imaging densitometry method for the determination of phospholipid content. FTIR was found to be a useful analytical tool for simple and rapid quantitative determination of phospholipids in vegetable oil.read more
Citations
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Journal ArticleDOI
Identification and quantification of valuable plant substances by IR and Raman spectroscopy
TL;DR: In this article, a review of infrared and Raman spectroscopic methods applied to the analysis of valuable plant substances or quality parameters in horticultural and agricultural crops is presented.
Journal ArticleDOI
New analytical techniques in food science.
Elena Ibáñez,Alejandro Cifuentes +1 more
TL;DR: Referee: Dr. Jerry King, 1820 Sunnyview Drive, Peoria, IL 61604
Book ChapterDOI
Determination of Food Quality by Using Spectroscopic Methods
TL;DR: The food quality includes also chemical, biological and microbial factors, e.g. instability of food products, which limits their shelf life and is connected with irreversible chemical and enzymatic reacclinations as mentioned in this paper.
Journal ArticleDOI
Vesicle size-dependent translocation of penetratin analogs across lipid membranes
TL;DR: Surprisingly, in contrast to the behavior in living cells, all peptides readily entered the giant vesicles as proved by confocal microscopy, while none of them could cross the membranes of LUVs (100 nm).
Journal ArticleDOI
Soy Lecithin Phospholipid Determination by Fourier Transform Infrared Spectroscopy and the Acid Digest/Arseno-Molybdate Method: A Comparative Study
J. M. Nzai,A. Proctor +1 more
TL;DR: In this paper, a study was conducted to determine the accuracy and precision of a simple Fourier transform infrared spectroscopy (FTIR) method relative to the conventional phospholipid phosphorus analysis by the acid digest/arseno-molybdate method by Bartlett.
References
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Journal ArticleDOI
Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
Journal ArticleDOI
Improved separation of phospholipids in thin layer chromatography
TL;DR: The mobile phases described permit separation of the six major phospholipids of amniotic fluid in one dimension with either conventional or high performance thin layer chromatography.
Journal ArticleDOI
Monitoring the oxidation of edible oils by fourier transform infrared spectroscopy.
TL;DR: In this paper, Fourier transform infrared (FTIR) spectroscopy was used for the analysis of Safflower and cottonseed oils under various conditions, and their spectral changes were recorded and interpreted.
Journal ArticleDOI
Quantitative determination of conformational disorder in the acyl chains of phospholipid bilayers by infrared spectroscopy.
TL;DR: A method is proposed and demonstrated for the direct determination of conformational disorder (trans-gauche isomerization) as a function of acyl-chain position in phospholipid bilayer membranes with significant advantages of the FT-IR approach.
Journal ArticleDOI
Quantitative determination of phospholipids in sunflower oil
TL;DR: In this paper, the results demonstrated the utility of SPE-HPLC for quantitative analysis of phospholipids in sunflower oil and the effectiveness for concentrating, separating, identifying, and quantitating phospholipsid in samples with phosphatide contents as low as 0.1%.
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Soy Lecithin Phospholipid Determination by Fourier Transform Infrared Spectroscopy and the Acid Digest/Arseno-Molybdate Method: A Comparative Study
J. M. Nzai,A. Proctor +1 more