Journal ArticleDOI
Direct organogenesis from hypocotyl-derived explants of annatto ( Bixa orellana )
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TLDR
Histological investigation showed that zeatin-induced meristemoids originated mainly from wounding tissues, and that TDZ induced a high level of mitotic division resulting in several proliferation zones nearby the epidermis and outer cortical tissues.Abstract:
The relative importance of explant, cytokinin type, carbon source and gelling agent for annatto organogenesis was studied The best organogenic response, including adventitious shoot number and elongation, was obtained when hypocotyl segments and rooted hypocotyls were cultured onto MS medium supplemented with 456 μM zeatin, 876 mM sucrose, and 28 g l−1 Phytagel® Adventitious shoots derived from hypocotyl segments were less frequent and more difficult to elongate than those derived from rooted hypocotyls Thidiazuron (TDZ) promoted a higher organogenic response in rooted hypocotyls, resembling a rosette-like structure, but impaired shoot elongation Histological investigation showed that zeatin-induced meristemoids originated mainly from wounding tissues, and that TDZ induced a high level of mitotic division resulting in several proliferation zones nearby the epidermis and outer cortical tissues Rhizogenesis efficiency (rooting frequency and root number) was greater at the highest indole-3-butyric acid (IBA) concentration (50 μM) employed, although calli occurred at the basal end of shoots Eighty percent of rooted plantlets survived after acclimatization This optimized regeneration protocol may enable further development of an efficient genetic transformation protocol for this speciesread more
Citations
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Journal ArticleDOI
An efficient micropropagation system for Eclipta, alba —A valuable medicinal herb
Ponnusamy Baskaran,N. Jayabalan +1 more
TL;DR: Micropropagated plants established in garden soil, farmyard soil, and sand were uniform and identical to the donor plant with respect to growth characteristics as well as floral features.
BookDOI
Medicinal and Aromatic Plants of the World
TL;DR: MAPs have become “industrial products” with new concepts like phytotherapy and veterinary medicinal uses, aromatherapy, nutraceuticals, cosmeceuticals, and animal welfare uses widening the scope of the utilization.
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Callus induction and plant regeneration in Cardiospermum halicacabum Linn. an important medicinal plant
T. Dennis Thomas,E.A. Maseena +1 more
TL;DR: In vitro propagation protocol for rapid micropropagation of C. helicacabum through plant regeneration from leaf and nodal explant derived calli has been developed and should be useful for conservation as well as mass propagation of this medicinal plant.
Journal ArticleDOI
Enhanced shoot organogenesis in Bixa orellana L. in the presence of putrescine and silver nitrate
TL;DR: An efficient protocol for direct shoot organogenesis in Bixa orellana, known as achiote or annatto or Latkhan (India), has been developed and can be used for improving the in vitro regeneration of B. Orellana for transformation studies.
Journal ArticleDOI
Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant
A. Shahanaz Beegum,K. P. Martin,Chun-Lai Zhang,I. K. Nishitha,Ligimol,Adrian Slater,P. V. Madhusoodanan +6 more
TL;DR: Procedures for the rapid, high frequency regeneration of a camptothecin producing plant, Ophiorrhiza prostrata D, are presented via shoot organogenesis and the concentrations of plant growth regulators and explant types exhibited discrete roles in the efficacy of shoot induction.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
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Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Thidiazuron: a potent cytokinin for woody plant tissue culture
Carl A. Huetteman,John E. Preece +1 more
TL;DR: Thidizuron (TDZ) is among the most active cytokinin-like substances for woody plant tissue culture and facilitates efficient micropropagation of many recalcitrant woody species.
Journal ArticleDOI
Polar auxin transport: controlling where and how much
Gloria K. Muday,Alison DeLong +1 more
TL;DR: New data indicate that reversible protein phosphorylation can control the amount of Auxin transport, whereas protein secretion through Golgi-derived vesicles and interactions with the actin cytoskeleton might regulate the localization of auxin efflux complexes.
Journal ArticleDOI
The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora
Sandra R.L. Fuentes,Maria Beatriz P. Calheiros,João Manetti-Filho,Luiz Gonzaga Esteves Vieira +3 more
TL;DR: At concentrations between 30–60 μM, AgNO3 improved embryo yield for the genotypes evaluated, while higher doses negatively affected the regenerative capacity, and the substitution of maltose, glucose or fructose for sucrose produced different responses depending on the genotype.
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