Journal ArticleDOI
Directed evolution to generate cycloartenol synthase mutants that produce lanosterol.
TLDR
Direct evolution was used to find cycloartenol synthase residues that affect cyclopropyl ring formation, selecting randomly generated cycloarternal synthase mutants for their ability to genetically complement a yeast strain lacking lanosterol synth enzyme.About:
This article is published in Organic Letters.The article was published on 2002-03-19. It has received 67 citations till now. The article focuses on the topics: Cycloartenol synthase & Lanosterol.read more
Citations
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Sterols in microorganisms.
TL;DR: This review describes the variety of sterol structures found in microalgae, yeasts, fungi, protozoans and microheterotrophs, and possible application areas for sterols derived from mass culture of micro algae and other microorganisms are highlighted.
Journal ArticleDOI
Catalytic Promiscuity in Biocatalysis: Using Old Enzymes to Form New Bonds and Follow New Pathways
TL;DR: Combined with protein engineering, the catalytic promiscuity of enzymes may broadly extend their usefulness in organic synthesis.
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On the origins of triterpenoid skeletal diversity.
TL;DR: The triterpenoids are a large group of natural products derived from C(30) precursors and each of these structures is classified and mechanisms for their formation are provided.
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Biogenesis, molecular regulation and function of plant isoprenoids
TL;DR: The function and evolutionary implications of this metabolism are discussed in relation with seminal informations gathered from distantly but related organisms.
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The role of sterols in plant growth and development.
TL;DR: The characterization of genes encoding sterol biosynthetic enzymes and the isolation of novel plant lines affected in the expression of those genes have shed new light on the involvement of sterols in biological processes such as embryonic development, cell and plant growth, and fertility.
References
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Journal ArticleDOI
Structural Basis for Cyclic Terpene Biosynthesis by Tobacco 5-Epi-Aristolochene Synthase
Courtney M. Starks,Kyoungwhan Back,Kyoungwhan Back,Joseph Chappell,Joseph Chappell,Joseph P. Noel,Joseph P. Noel +6 more
TL;DR: These structures reveal an unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the stereochemical selectivity displayed by other cyclases in the biosynthesis of pharmacologically important cyclic terpenes.
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Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes
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Structure and function of a squalene cyclase
TL;DR: The crystal structure of squalene-hopene cyclase from Alicyclobacillus acidocaldarius was determined and reveals a membrane protein with membrane-binding characteristics similar to those of prostaglandin-H2 synthase, the only other reported protein of this type.
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Beta-amyrin synthase--cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants.
TL;DR: The present studies have revealed that distinct OSC exists for triterpene formation in higher plants, and the high level of similarity with cycloartenol synthase indicates close evolutional relationship between sterol and triterPene biosynthesis.
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Isolation of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen
TL;DR: The cloning and characterization of CAS1, an Arabidopsis thaliana gene encoding cycloartenol synthase, is reported, which should be generally applicable to genes responsible for secondary metabolite biosynthesis.