Journal ArticleDOI
Directed transposition of the arabinose operon: A technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene☆☆☆
TLDR
An F-thr-ara ‡ episome has been inserted into the Escherichia coli chromosome at the T1 locus near the attachment site for bacteriophage φ 80 and a φ80dara transducing phage was isolated from a strain carrying such an insertion.About:
This article is published in Journal of Molecular Biology.The article was published on 1969-08-28. It has received 137 citations till now. The article focuses on the topics: Bacteriophage & L-arabinose operon.read more
Citations
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Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
TL;DR: Translocatable drug-resistance elements are useful in isolation of mutants, in the construction of strains and other genetic manipulations (even when selection is difficult or impossible), in localized mutagenesis, in chromosomal mapping, in construction of Hfr strains with known origin and direction of chromosome transfer, in complementation tests.
Journal ArticleDOI
Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages.
Edith Brickman,Jon Beckwith +1 more
TL;DR: π�80 transducing phages for the proC †, phoA and phoB genes of Escherichia coli have been obtained, in agreement with a positive control mechanism for the regulation of alkaline phosphatase synthesis.
Journal ArticleDOI
E. coli mutant pleiotropically defective in the export of secreted proteins
Donald Oliver,Jon Beckwith +1 more
TL;DR: A temperature-sensitive conditional lethal mutant is isolated that accumulates the precursor of the maltose-binding protein in the cytoplasm, and also accumulates precursors of alkaline phosphatase, lambda receptor protein and the ompF gene gene product.
Journal ArticleDOI
Genetic studies of the lac repressor
TL;DR: Schmeissner et al. as mentioned in this paper used a fine structure deletion map to construct a combined gene-protein map, in which deletion intervals on the genetic map are correlated with known segments of the protein sequence.
References
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Journal ArticleDOI
Genetic regulatory mechanisms in the synthesis of proteins.
François Jacob,Jacques Monod +1 more
TL;DR: The synthesis of enzymes in bacteria follows a double genetic control, which appears to operate directly at the level of the synthesis by the gene of a shortlived intermediate, or messenger, which becomes associated with the ribosomes where protein synthesis takes place.
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Transduction of linked genetic characters of the host by bacteriophage P1
TL;DR: Transduction of characters between bacteria of the coli and dysentery groups indicates genetic homologies between these groups.
Journal ArticleDOI
The genetic control and cytoplasmic expression of “Inducibility” in the synthesis of β-galactosidase by E. coli
TL;DR: The study of galactosidase synthesis in heteromerozygotes of E. coli indicates that the z and i mutations belong to different cistrons, and the kinetics of expression of the i + (inducible) character suggest that the i gene controls the synthesis of a specific substance which represses the synthesis.
Journal ArticleDOI
Transducing fragments in generalized transduction by phage P1 I. Molecular origin of the fragments
Hideo Ikeda,Jun-ichi Tomizawa +1 more
TL;DR: The analysis of the phage yield by CsCl density-gradient centrifugation showed that the transducing particles carrying various chromosomal genes have a density and band profile similar to those prepared on bromouracil-labelled bacteria in a medium containing bromouredacil.