Journal ArticleDOI
DNA biosynthesis in mitochondria: Differential inhibition of mitochondrial and nuclear DNA polymerases by the mutagenic dyes ethidium bromide and acriflavin
Ralph R. Meyer,Melvin V. Simpson +1 more
TLDR
The DNA-intercalating dyes ethidium bromide and acriflavin strongly inhibit the enzymatic synthesis of DNA catalyzed by rat liver mitochondrial DNA polymerase, and the mitochondrial enzyme is much more sensitive to these dyes.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1969-01-27. It has received 146 citations till now. The article focuses on the topics: Ethidium bromide & Polymerase.read more
Citations
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Journal ArticleDOI
The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide.
TL;DR: The mechanism of this conversion in Saccharomyces cerevisiae is investigated by examining the properties of mitochondrial DNA at various times during the mutation process, using cycloheximide to amplify the proportion of radioactivity in mitochondrial DNA.
Book ChapterDOI
The Kinetoplast of the Hernoflagellates
TL;DR: The existence of one mitochondrion per cell should be conclusively proved by the three-dimensional reconstruction technique and the replication of K-DNA should be investigated, both in terms of the intracellular regulatory mechanisms involved in the synchrony of the kinetoplast S period with the nuclear S period and the actual replication of the minicircles and long DNA molecules.
Book ChapterDOI
Use of Ethidium Bromide for Separation and Determination of Nucleic Acids of Various Conformational Forms and Measurement of Their Associated Enzymes
Journal ArticleDOI
Deoxyribonucleic acid biosynthesis in mitochondria. Purification and general properties of rat liver mitochondrial deoxyribonucleic acid polymerase.
Ralph R. Meyer,Melvin V. Simpson +1 more
TL;DR: The purified Deoxyribonucleic acid polymerase has been purified 3550-fold from rat liver mitochondria, which computes to 14,000-fold based on whole liver as the starting material.
References
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Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Journal ArticleDOI
A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.
J.-B. Lepecq,Claude Paoletti +1 more
TL;DR: Evidence is presented that formation of this complex (complex I) is specific for base-paired regions either in DNA, RNA or RNA: DNA hybrids, and that the basis of this specificity is intercalation of the dye between base pairs.
Journal ArticleDOI