Journal ArticleDOI
DNA-polymerases in neuron and glial cells of developing and aging mouse brain.
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TLDR
DNA‐polymerase‐β remained high throughout the period studied though a second peak of activity was also observzd at day 30 in both cell types, suggesting a possible role in DNA‐replication in addition to DNA‐repair.Abstract:
DNA-polymerases alpha and beta were studied in neuron and glial cells of mouse during developing and aging brain Maximum activity of alpha-enzyme was found to be prenatal, though a very low but significant level could be detected in aging brain Furthermore, this pattern was found to be dependent upon cell types that shift in peak activity just before birth in glial cells in contrast to neurons DNA-polymerase-beta remained high throughout the period studied though a second peak of activity was also observed at day 30 in both cell types, suggesting a possible role in DNA-replication in addition to DNA-repair It was found that beta-enzyme from glial cells behaves differently than the same enzyme from neuronsread more
Citations
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Journal ArticleDOI
Manganese neurotoxicity: cellular effects and blood-brain barrier transport.
Michael Aschner,Judy L. Aschner +1 more
TL;DR: The first part of this review summarizes the current understanding of the interaction of Mn with biologically active sites in the induction of Mn cytotoxicity, with the contention that the rate and extent of Mn transport across the blood-brain barrier modulates its neurotoxicity.
Book ChapterDOI
Biological significance of manganese in mammalian systems.
TL;DR: This chapter discusses biological significance of manganese in mammalian systems and describes the limited number of redox enzymes reported to involve Mn(II) or Mn(III) directly in catalysis, all of which utilize one-electron redox reactions.
Journal ArticleDOI
Base excision repair and the central nervous system.
TL;DR: What is known about BER protein function(s) in the CNS, which in cooperation with the peripheral nervous system operates to control physical responses, motor coordination, and brain operation, is highlighted.
Journal ArticleDOI
Unscheduled DNA synthesis in various types of cells of the mouse brain in vivo.
H. Korr,B. Schultze +1 more
TL;DR: Very low incorporation of 3H-thymidine (TdR) into neurons and non-proliferating glial and endothelial cells in various brain areas of the adult mouse after3H- TdR injection and subsequent X-irradiation of the head with 45 Gy has been demonstrated autoradiographically after exposure times of 250 days.
Journal ArticleDOI
DNA-polymerase α, β, δ and ε activities in isolated neuronal and astroglial cell fractions from developing and aging rat cerebral cortex
TL;DR: A notable difference regarding the relative abundance of DNA‐polymerases other than β is the higher percentage of pol δ/ε in neurons and a more sustained pol α activity through the life span in astroglia.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues.
TL;DR: The methods used at present for the determination of the higher nucleic acids in organs are based on certain color reactions of their carbohydrate components, but if the quantitative estimations of desoxyribonucleic acid and of ribon nucleic acid could be based on phosphorus determinations rather than on color tests of their carbohydrates components, these difficulties might be avoided.
Journal ArticleDOI
Autoradiographic and histological studies of postnatal neurogenesis. I. A longitudinal investigation of the kinetics, migration and transformation of cells incorporating tritiated thymidine in neonate rats, with special reference to postnatal neurogenesis in some brain regions.
Joseph Altman,Gopal D. Das +1 more
TL;DR: It was established that cells multiplying in the ependymal and subependymal walls of the Olfactory ventricle migrate outward into the olfactory bulb, where they become differentiated into granule cells, and, to a lesser extent, other types of nerve cells of the cerebellar cortex.
Book ChapterDOI
Mammalian DNA Polymerases
TL;DR: It is stated that physiological experiments demonstrating variations of DNA polymerase activities were first done in mammalian systems, whereas the first relevant genetic experiments in bacteria came 12 years after the discovery of Escherichia coliDNA polymerase I.
Journal ArticleDOI
Induction of DNA polymerase in mouse L cells.
TL;DR: Two molecular species of DNA polymerase are found in mouse L cells and the variation of these two species of enzyme with the rate of cell growth and DNA synthesis is concerned.