Journal ArticleDOI
DNA recovery and PCR quantification of catechol 2,3-dioxygenase genes from different soil types
Reads0
Chats0
TLDR
The concentration of PAHs and catechol 2,3-dioxygenase DNA in PAH-contaminated sediment undergoing an ex situ compost process was quantified by competitive PCR over a period of 16 weeks and was found to correlate.About:
This article is published in Journal of Biotechnology.The article was published on 1996-12-10. It has received 79 citations till now. The article focuses on the topics: DNA extraction & Polymerase chain reaction.read more
Citations
More filters
Journal ArticleDOI
Utilising the Synergy between Plants and Rhizosphere Microorganisms to Enhance Breakdown of Organic Pollutants in the Environment (15 pp)
TL;DR: Plant-microbial interactions in the rhizosphere offer very useful means for remediating environments contaminated with recalcitrant organic compounds and will provide a basis for improving the efficacy of biological remediations.
Journal ArticleDOI
Molecular analysis of ammonia oxidation and denitrification in natural environments
TL;DR: This review summarizes aspects of the current knowledge about the ecology of ammonia-oxidizing and denitrifying bacteria and indicates that the composition of nitrifying and denItrifying communities is complex and apparently subject to large fluctuations, both in time and in space.
Journal ArticleDOI
Molecular Techniques for Determining Microbial Diversity and Community Structure in Natural Environments
Jacques Theron,Thomas E. Cloete +1 more
TL;DR: Recombinant DNA techniques have provided a means whereby many of the obstacles associated with cultivation and description can be overcome and subsequently has allowed many new insights into the complexity of natural microbial communities.
Journal ArticleDOI
A strategy for optimizing quality and quantity of DNA extracted from soil
TL;DR: This study provides detailed guidelines on how to optimize the general method to obtain optimal DNA from individual soils, and proposes a protocol for the quantification of the total DNA content in soils.
Journal ArticleDOI
Evaluation of extraction and purification methods for obtaining PCR-amplifiable DNA from compost for microbial community analysis
TL;DR: Combinations of DNA extraction and purification methods were compared based on DNA yield, humic acid contamination, PCR amplifiability, and microbial community structure assessed by terminal restriction fragment length polymorphisms (TRFLP) of amplified 16S rRNA genes to identify patterns that were not consistent and introduced a bias towards lower estimates of diversity.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
STUDIES ON LYSOGENESIS I.: The Mode of Phage Liberation by Lysogenic Escherichia coli
TL;DR: Observations on a lysogenic strain of Escherichia coli that carries more than one detectable type of phage that is associated with bacteriophage infection followed by lysis of the infected cell are reported.
Journal ArticleDOI
DNA recovery from soils of diverse composition.
TL;DR: Four methods for purifying crude DNA were evaluated for percent recovery, fragment size, speed, enzyme restriction, PCR amplification, and DNA-DNA hybridization and in general, all methods produced DNA pure enough for PCR amplification.
Book
Molecular Microbial Ecology Manual
TL;DR: The state-of-the-art methods described in MMEM-II have not only been provided by experts in the field, but in most cases by the laboratories that actually first developed and applied the methods, thus providing the MM EM-II user with unique first-hand tips and insight.
Journal ArticleDOI
Bacterial gene transfer by natural genetic transformation in the environment.
TL;DR: The current understanding of the biology of transformation is summarized to provide the platform on which aspects of bacterial transformation in water, soil, and sediments and the habitat of pathogens are discussed.