DNAzol: a reagent for the rapid isolation of genomic DNA.
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TLDR
DNAzol, a patent-pending DNA isolation reagent containing guanidine thiocyanate and a detergent mixture, is a complete, nontoxic and ready-to-use reagent for the isolation of genomic DNA from various biological sources.Abstract:
In this report, we present DNAzol®, a patent-pending DNA isolation reagent containing guanidine thiocyanate and a detergent mixture. It is a complete, nontoxic and ready-to-use reagent for the isol...read more
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Maternal Methyl Supplements in Mice Affect Epigenetic Variation and DNA Methylation of Offspring
Craig A. Cooney,Craig A. Cooney,Apurva A. Dave,Apurva A. Dave,George Louis Wolff,George Louis Wolff +5 more
TL;DR: Methyl supplements increase the level of DNA methylation in the agouti LTR and change the phenotype of offspring in the healthy, longer-lived direction, which shows that methyl supplements have strong effects onDNA methylation and phenotype and are likely to affect long-term health.
Journal ArticleDOI
Molecular systematics of cowries (Gastropoda: Cypraeidae) and diversification patterns in the tropics
TL;DR: A strong phylogenetic trend supports these observations as lineages leading up to and including the derived Indo-West Pacific Erroneinae clade contain taxa predominantly restricted to continental habitats and have undergone the greatest evolutionary radiations in their respective regions.
Journal ArticleDOI
Medusozoan Phylogeny and Character Evolution Clarified by New Large and Small Subunit rDNA Data and an Assessment of the Utility of Phylogenetic Mixture Models
Allen Gilbert Collins,Peter Schuchert,Antonio C. Marques,Thomas Jankowski,Mónica Medina,Bernd Schierwater +5 more
TL;DR: A newly compiled data set of nearly complete sequences of the large subunit of the nuclear ribosome (LSU or 28S) sampled from 31 diverse medusozoans greatly clarifies the phylogenetic history of Cnidaria.
Journal ArticleDOI
Phylogeny of Medusozoa and the evolution of cnidarian life cycles
TL;DR: To investigate the evolution of cnidarian life cycles, data from the small subunit of the ribosome are used and indicate that Cnidaria is monophyletic and composed of Anthozoa and Medusozoa.
Journal ArticleDOI
Evaluation of Five Methods for Total DNA Extraction from Western Corn Rootworm Beetles
TL;DR: These evaluations provide a guide for choosing methods of DNA extraction from western corn rootworm beetles based on expected DNA yield and quality, extraction time, cost, and waste control.
References
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Rapid isolation of eukaryotic DNA.
TL;DR: Intact and readily digestible DNA was prepared from a range of murine tissues and cell lines which had been dispersed in GHCl and ethanol precipitated, and then rinsed in 70% ethanol and resuspended in 10 mM Tris-HCl, 1 mM EDTA, pH 7.5.
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One-hour downward capillary blotting of RNA at neutral pH.
Piotr Chomczynski,Karol Mackey +1 more
TL;DR: This report describes a setup for the downward capillary blotting of RNA with the use of 10 x SSC as a transfer solution, composed of a stack of blotting papers, hybridization membrane, and agarose gel.