Journal ArticleDOI
Double-Label Immunofluorescence with the Laser Scanning Confocal Microscope Using Cyanine Dyes
TLDR
The laser scanning confocal microscope, when used with the krypton-argon ion laser, is well suited for the simultaneous detection of pairs of antigens by immunofluorescence, but the use of fluorophores excited by the 488 nm line produces unsatisfactory results when tissue contains low wavelength-excitable aut ofluorescence.About:
This article is published in NeuroImage.The article was published on 1994-11-01. It has received 27 citations till now. The article focuses on the topics: Texas Red & Autofluorescence.read more
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Journal ArticleDOI
Characterization of Photoinduced Isomerization and Back-Isomerization of the Cyanine Dye Cy5 by Fluorescence Correlation Spectroscopy
Jerker Widengren,Petra Schwille +1 more
TL;DR: In this article, the fluorescence fluctuations of Cy5 have been investigated under steady-state excitation conditions by fluorescence correlation spectroscopy (FCS), which can be used to characterize the photoinduced isomerization and back-isomerization, as well as the transitions between the singlet and triplet states.
Journal ArticleDOI
Perisynaptic surface distribution of multiple classes of nicotinic acetylcholine receptors on neurons in the chicken ciliary ganglion
HL Horch,Peter B. Sargent +1 more
TL;DR: The results suggest that most surface AChRs in both embryonic and mature chicken ciliary neurons are perisynaptic, which raises questions about the function of these AChR classes.
Journal ArticleDOI
Human Muscarinic Cholinergic Receptor Hm1 Internalizes via Clathrin-coated Vesicles
Lara M. Tolbert,Jelveh Lameh +1 more
TL;DR: It is shown that agonist-induced internalization of the Hm1 receptor occurs via clathrin-coated vesicles in HEK cells, and caveolin, the protein that comprises caveolae, did not colocalize with Hm 1 in intracellular vesicle following agonist treatment, indicating that Caveolae are not involved in the agonist -inducedinternalization of Hm2.
Journal ArticleDOI
A Constitutively Internalizing and Recycling Mutant of the μ-Opioid Receptor
TL;DR: It is demonstrated that a mutant GPCR can be constructed such that internalization, normally an agonist‐dependent process, can occur spontaneously without concomitant G protein activation.
Journal ArticleDOI
Pronounced cellular diversity and extrasynaptic location of nicotinic acetylcholine receptor subunit immunoreactivities in the chicken pretectum
Erik M. Ullian,Peter B. Sargent +1 more
TL;DR: The pronounced diversity of A ChR subunit expression and the extrasynaptic location of AChR-LI suggest that AChr-like molecules in the SpL do not function solely to respond to transmitter focally released from presynaptic terminals.
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