Effect of incorporating additional oleic acid into the plasma membrane of baker's yeast on the permeation of pyruvic acid
TLDR
Adding Tween 80 to the growth medium of baker's yeast under anaerobic conditions resulted in an increased content of 9-cis-octadecenoic acid (oleic acid) in a membrane preparation consisting mainly of plasma membrane, presumably because of an oleate-induced increase in the permeability of the plasma membrane to pyruvate.Abstract:
Addition of Tween 80 to the growth medium of baker's yeast (Saccharomyces cerevisiae) under anaerobic conditions resulted in an increased content of 9-cis-octadecenoic acid (oleic acid) in a membrane preparation consisting mainly of plasma membrane. There was no significant effect on the pyruvate decarboxylation capacity of disintegrated cells, but that of intact cells was significantly enhanced, presumably because of an oleate-induced increase in the permeability of the plasma membrane to pyruvate.read more
Citations
More filters
Journal ArticleDOI
Ethanol and the fluidity of the yeast plasma membrane
Rod Jones,Paul F. Greenfield +1 more
TL;DR: The concept that increased fluidity is an adaptive response conferring ethanol tolerance is disputed and an alternative hypothesis, namely that the observed increase in fluidity was the net result of a number of more fundamental changes, is presented to explain the observed effects.
Journal ArticleDOI
Involvement of lipids in solute transport in yeasts.
Rajendra Prasad,Anthony H. Rose +1 more
Journal ArticleDOI
Permeability and membrane sterol distribution in Saccharomyces uvarum and Kluyveromyces bulgaricus grown in presence of polyoxyalkylene glycol-oleic acid condensates
TL;DR: Antifoam agents, such as polyoxyalkylene glycol-oleic acid condensates, increase cell permeability in Saccharomyces uvarum, but decrease cell permeable in Kluyveromyces bulgaricus.
Book ChapterDOI
Lipids in the structure and function of yeast membrane.
TL;DR: It is apparent that yeast lipids regulate many membrane functions and also have decisive involvement in its structure, and a multidisciplinary approach is required where both its physical and physiological parameters should be simultaneously studied.
Book ChapterDOI
Membrane Lipid Adaptation in Yeast
TL;DR: It is well recognized that the lipid composition of yeast varies with the growth conditions, and the reviews by Hunter and Rose (1971) and Rattray et al. (1975) should be consulted for references prior to 1975.
References
More filters
Journal ArticleDOI
Anaerobic nutrition of saccharomyces cerevisiae. II. Unsaturated fatty and requirement for growth in a defined medium
A. A. Andreasen,T. J. B. Stier +1 more
Journal ArticleDOI
Gas Chromatography of Barbiturates, Phenolic Alkaloids, and Xanthine Bases: Flash-Heater Methylation by Means of Trimethylanilinium Hydroxide
TL;DR: Injection of methanol solutions of trimethylanilinium salts of barbiturates, phenolic alkaloids, and dimethylxanthines produced thermal decomposition in the injection port to give methyl derivatives suitable for quantitative gas chromatography.
Journal ArticleDOI
Composition of the protoplast membrane from Saccharomyces cerevisiae.
TL;DR: Phosphorus analyses on phospholipid fractions from membranes and whole cells showed that membranes contained proportionately more phosphatidylethanolamine and phosph atidylinositol+phosphatidylserine than whole cells, which in turn were richer in phosphatodylcholine.
Journal ArticleDOI
The enzymic composition of the isolated cell wall and plasma membrane of baker's yeast
TL;DR: A study was made of the enzyme content of the isolated cell walls and of a plasma-membrane preparation obtained by centrifugation after enzymic digestion of the cell walls of baker's yeast, finding it may be assumed that the enzymes released into the medium during digestion are located in the cell wall outside the plasma membrane.
Journal ArticleDOI
Osmotic lysis of sphaeroplasts from Saccharomyces cerevisiae grown anaerobically in media containing different unsaturated fatty acids
F. Alterthum,A. H. Rose +1 more
TL;DR: In this article, the authors showed that Sphaeroplasts formed from organisms grown in oleic acid-containing medium were less susceptible to osmotic lysis than those formed from these organisms in the absence of spermine.
Related Papers (5)
Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasma-membrane lipid composition.
Thomas Ds,Rose Ah +1 more
Cell permeability and decarboxylation of α-keto acids by intact yeast
Heikki Suomalainen,Erkki Oura +1 more