Effect of Neem Leaf Extract and Neem Oil on Penicillium Growth, Sporulation, Morphology and Ochratoxin A Production
TLDR
Given its accessibility and low cost, neem oil could be implemented as part of a sustainable integrated pest management strategy for plant disease, as it has been shown to be fungitoxic by inhibition of growth and sporulation.Abstract:
In vitro trials were conducted to evaluate the effect of Azadirachta indica (neem) extracts on mycelial growth, sporulation, morphology and ochratoxin A production by P. verrucosum and P. brevicompactum. The effect of neem oil extract from seeds and leaf was evaluated at 0.125; 0.25 and 0.5% and 6.25 and 12.5 mg/mL, respectively, in Yeast Extract Sucrose (YES) medium. Ochratoxin A production was evaluated by a thin-layer chromatography technique. Oil extracts exhibited significant (p ≤ 0.05) reduction of growth and sporulation of the fungi. No inhibition of ochratoxin A production was observed. Given its accessibility and low cost, neem oil could be implemented as part of a sustainable integrated pest management strategy for plant disease, as it has been shown to be fungitoxic by inhibition of growth and sporulation.read more
Citations
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References
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John I. Pitt,Ailsa D. Hocking +1 more
TL;DR: The Ecology of Fungal Food Spoilage: Naming and Classifying Fungi and Methods for Isolation, Enumeration and Identification is presented.
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Journal ArticleDOI
An easy screening method for fungi producing ochratoxin A in pure culture
TL;DR: A simple screening method has been developed for detecting ochratoxin production by fungi, based on high-performance liquid chromatographic determinations on extracts obtained from agar plugs cut from pure Petri dish cultures, which provides quantitative information on the level of ochRatoxin produced by the cultures.
Journal ArticleDOI
Classification of terverticillate penicillia based on profiles of mycotoxins and other secondary metabolites
J C Frisvad,O Filtenborg +1 more
TL;DR: Strains of available terverticillate penicillium species and varieties were analyzed for profiles of known mycotoxins and other secondary metabolites produced on Czapek yeast autolysate agar and yeast extract-sucrose agar by using simple thin-layer chromatography screening techniques.
Journal ArticleDOI
Inhibitory effects of spices on growth and toxin production of toxigenic fungi.
TL;DR: The inhibitory effects of 29 commercial powdered spices on the growth and toxin production of three species of toxigenic Aspergillus were observed by introducing these materials into culture media for mycotoxin production.