Journal ArticleDOI
Effect of plant growth regulators on the cellular growth and levels of intracellular protein, starch and polyamines in embryogenic suspension cultures of Pinus taeda
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TLDR
Investigation of cell growth dynamics, intracellular levels of proteins, starch and polyamines in suspension cultures of Pinus taeda established in plant growth regulator-free medium and in medium supplemented with 2 μM 2,4-dichlorophenoxyacetic acid and 0.5 μM Kinetin finds highest putrescine levels occurred in cultures growing in BM0 medium and this was associated with the low cellular growth.Abstract:
Somatic embryogenesis is the most important in vitro culture system for conifer propagation. However, Pinus taeda has been considered recalcitrant to somatic embryogenesis in commercial scale-up. The study of biochemical and physiological aspects of cell growth could lead to a better understanding of somatic embryogenesis in this species. In the present work, we investigated the cell growth dynamics, intracellular levels of proteins, starch and polyamines in suspension cultures of Pinus taeda established in plant growth regulator-free medium (BM0) and in medium supplemented with 2 μM 2,4-dichlorophenoxyacetic acid, 0.5 μM 6-benzylaminopurine and 0.5 μM Kinetin (BM2). Cell cultures growing in BM0 medium showed an increase in the sedimented cell volume from 3.77 to 17.73 ml after 24 days of culture. Those cultured in BM2 medium showed an increase in the sedimented cell volume from 4.23 to 25.17 ml after 20 days of culture. Intracellular proteins levels increased during the exponential growth phase and starch levels decreased until the exponential phase, followed by a synthesis up to the stationary phase, in both BM0 and BM2 media. Highest putrescine levels occurred in cultures growing in BM0 medium and this was associated with the low cellular growth.read more
Citations
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Journal ArticleDOI
Polyamines Induce Rapid Biosynthesis of Nitric Oxide (NO) in Arabidopsis thaliana Seedlings
Ni Ni Tun,Claudete Santa-Catarina,Tahmina Begum,Vanildo Silveira,Walter Handro,Eny Iochevet Segal Floh,Günther F. E. Scherer +6 more
TL;DR: It is observed that PAs induced NO biosynthesis in specific tissues in Arabidopsis seedlings, and spermine, the most active PA, stimulated NO release with no apparent lag phase.
Journal ArticleDOI
Polyamines, polyamine oxidases and nitric oxide in development, abiotic and biotic stresses
TL;DR: Exogenous application of PAs putrescine, spermidine and spermine to Arabidopsis seedlings induced NO production as observed by fluorimetry and fluorescence microscopy and pave a new insight into PA-mediated signalling and NO as a potential mediator of PA actions.
Journal ArticleDOI
Polyamine effects on the endogenous polyamine contents, nitric oxide release, growth and differentiation of embryogenic suspension cultures of Araucaria angustifolia (Bert.) O. Ktze.
Vanildo Silveira,Claudete Santa-Catarina,Ni Ni Tun,Günther F. E. Scherer,Walter Handro,Miguel Pedro Guerra,Eny Iochevet Segal Floh +6 more
TL;DR: It was observed that embryonic cells of A. angustifolia accumulated more NO than the suspensor cells, suggesting that NO biosynthesis might be related to the maintenance of the polarity (embryonic–suspensor cells) present at the PEM II stage.
Journal ArticleDOI
Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures
Neusa Steiner,Claudete Santa-Catarina,Vanildo Silveira,Eny Iochevet Segal Floh,Miguel Pedro Guerra +4 more
TL;DR: PAs supplemented to a PGR-free BM medium, mainly Put and Spm, increased the endogenous IAA and ABA levels, showing a direct relationship -between PAs levels and A BA accumulation.
Journal ArticleDOI
IAA, ABA, polyamines and free amino acids associated with zygotic embryo development of Ocotea catharinensis
Claudete Santa-Catarina,Vanildo Silveira,Tiago Santana Balbuena,Ana Maria Viana,Maria Emília Maranhão Estelita,Walter Handro,Eny Iochevet Segal Floh +6 more
TL;DR: Results show that changes in the phytohormones (IAA, ABA and PAs) concentrations in combination with amino acids are likely important factors determining the developmental stages of O.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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TL;DR: The Anatomy and Morphology of Tissue Cultured Plants M.V. Moshkov, G. V. Novikova, M. Stasolla, E. Zazimalova and E.F. George reveal the secrets of successful tissue culture and the challenges faced in implementing and sustaining such a system.
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Determination of Starch and Amylose in Vegetables
Journal ArticleDOI
Polyamines and environmental challenges : recent development
TL;DR: All the data support the view that putrescine and derived polyamines (spermidine, spermine, long-chained polyamides) may have several functions during environmental challenges.
Journal ArticleDOI
Developmental pathways of somatic embryogenesis
TL;DR: It has long been observed that conditioned medium from embryogenic cultures can promote embryogenesis, and it is possible to make a model of the process through the construction of fate maps representing an adequate number of morphological and molecular markers.
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