Efficient and Specific Removal of Albumin from Human Serum Samples
Laura F. Steel,Michael G. Trotter,Pamela B. Nakajima,Taj S. Mattu,Gregory E. Gonye,Timothy M. Block +5 more
Reads0
Chats0
TLDR
Using monoclonal antibodies against human serum albumin (HSA) to develop an immunoaffinity resin that is effective in the removal of both full-length HSA and many of the HSA fragments present in serum shows markedly better performance than dye-based resins in terms of both the efficiency and specificity of albumin removal.About:
This article is published in Molecular & Cellular Proteomics.The article was published on 2003-04-01 and is currently open access. It has received 222 citations till now. The article focuses on the topics: Bovine serum albumin & Human serum albumin.read more
Citations
More filters
Journal ArticleDOI
Human serum albumin: from bench to bedside.
TL;DR: HSA is a valuable biomarker of many diseases, including cancer, rheumatoid arthritis, ischemia, post-menopausal obesity, severe acute graft-versus-host disease, and diseases that need monitoring of the glycemic control.
Journal ArticleDOI
Human body fluid proteome analysis
TL;DR: The proteomics technologies currently used for global identification and quantification of body fluid proteins are summarized, and the putative biomarkers discovered for a variety of human diseases through human body fluid proteome (HBFP) analysis are elaborate.
Journal ArticleDOI
Comparison of different depletion strategies for improved resolution in proteomic analysis of human serum samples.
TL;DR: This study provides a comprehensive investigation of commercially available depletion columns and will be of high importance for future proteomic studies on serum samples and the ProteoExtract™ Albumin/IgG Removal kit displayed the best results.
Journal ArticleDOI
Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma.
TL;DR: Non2‐D methods such as protein array pixelation are more promising strategies for detecting lower abundance proteins after depleting the six abundant proteins.
Journal ArticleDOI
Utilizing Human Blood Plasma for Proteomic Biomarker Discovery
Jon M. Jacobs,Joshua N. Adkins,Wei-Jun Qian,Tao Liu,Yufeng Shen,David G. Camp,Richard D. Smith +6 more
TL;DR: Continued development of depletion and enrichment techniques, coupled with improved pre-MS separations (both at the protein and peptide level) holds promise in extending the dynamic range of proteomic analysis.
References
More filters
Journal ArticleDOI
Probability-based protein identification by searching sequence databases using mass spectrometry data.
TL;DR: A new computer program, Mascot, is presented, which integrates all three types of search for protein identification by searching a sequence database using mass spectrometry data, and the scoring algorithm is probability based.
Journal ArticleDOI
The Human Plasma Proteome History, Character, and Diagnostic Prospects
TL;DR: This work speculates on the reasons behind this large discrepancy between the expectations arising from proteomics and the realities of clinical diagnostics and suggests approaches by which protein-disease associations may be more effectively translated into diagnostic tools in the future.
Journal ArticleDOI
Atomic structure and chemistry of human serum albumin.
Xiao Min He,Daniel C. Carter +1 more
TL;DR: The three-dimensional structure of human serum albumin has been determined crystallographically to a resolution of 2.8 Å and should provide insight into future pharmacokinetic and genetically engineered therapeutic applications of serumalbumin.
Book
Using Antibodies: A Laboratory Manual
Ed Harlow,David A. Lane +1 more
TL;DR: Epitope Mapping Determining the Structural Requirements Of An Epitope Choosing An EpITope-mapping Method Mapping By Competition Assay Mapping by Expression of Gene Fragments Mapping Using Synthetic Peptides Synthesis Or Purchase Of The Peptide Set Alternate Approaches To Epitopes.
Journal ArticleDOI
Erratum: Atomic structure and chemistry of human serum albumin
X. M. He,D. C. Carter +1 more
TL;DR: In this paper, the unit cell constant for the b axis of the recombinant crystal form should have been 88.3 A instead of 38.5 A, and a statement in Fig. 4 should read that Lys 199 and His 242 are located at the 'top' of Fig.