Electrically mediated delivery of plasmid DNA to the skin, using a multielectrode array.
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With the MEA, the muscle twitching associated with application of electric fields was notably reduced compared with conventional electrode systems, which will facilitate the translation of electroporation-mediated gene delivery to skin for clinical use with DNA vaccines or for therapies for cancer or protein deficiencies.Abstract:
The easy accessibility of skin makes it an excellent target for gene transfer protocols. To take full advantage of skin as a target for gene transfer, it is important to establish an efficient and reproducible delivery system. Electroporation is a strong candidate to meet this delivery criterion. Electroporation of the skin is a simple, direct, in vivo method to deliver genes for therapy. Previously, delivery to the skin was performed by means of applicators with relatively large distances between electrodes, resulting in significant muscle stimulation and pain. These applicators also had limitations in controlling the directionality of the applied field. To resolve this issue, a system consisting of an array of electrodes that decreased the distance between them and that were independently addressable for directional control of the field was developed. This new multielectrode array (MEA) was compared with an established electrode. In a rat model, comparable reporter expression was seen after delivery with each electrode. Delivery was also evaluated in a guinea pig model to determine the potential of this approach in an animal model with skin thickness and structure similar to human skin. The results clearly showed that effective delivery was related to both the electrode and the parameters chosen. With the MEA, the muscle twitching associated with application of electric fields was notably reduced compared with conventional electrode systems. This is important, as it will facilitate the translation of electroporation-mediated gene delivery to skin for clinical use with DNA vaccines or for therapies for cancer or protein deficiencies.read more
Citations
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Journal ArticleDOI
Clinical potential of electroporation for gene therapy and DNA vaccine delivery.
TL;DR: This review explores the electroporation mechanism and the parameters affecting its efficacy, and several of the possible optimization strategies for improving patient compliance and therapeutic efficacy are discussed.
Book ChapterDOI
Gene electrotransfer clinical trials.
Richard Heller,Loree C. Heller +1 more
TL;DR: This chapter focuses on clinical trials using in vivo electroporation or electrotransfer as a plasmid delivery method, and early clinical efficacy results are promising in both cancer therapeutic and infectious disease vaccine applications.
Book ChapterDOI
Electroporation-mediated gene delivery.
Jennifer L. Young,David A. Dean +1 more
TL;DR: This chapter will provide an overview of the theory of electroporation for the delivery of DNA both in individual cells and in tissues and its application for in vivo gene delivery in a number of animal models.
Journal ArticleDOI
Gene electrotransfer to skin; review of existing literature and clinical perspectives.
Anita Gothelf,Julie Gehl +1 more
TL;DR: The aim of this review is to summarize which plasmids were injected and the electrical parameters applied, and an overview of the clinical perspectives of gene electrotransfer to skin will be presented.
Journal ArticleDOI
Cell membrane electroporation-Part 3: the equipment
TL;DR: Electroporation of a cell membrane can be made permeable to various molecules by carrying out a procedure called electroporation, which requires electroporators and electrodes.
References
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Journal ArticleDOI
In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA.
TL;DR: In vivo electroporation can be used for the introduction of plasmid DNA into skin cells of mouse and NEO-resistant colonies were found in primary cell cultures obtained from the treated skin.
Journal ArticleDOI
In vivo electroporation for gene therapy.
Loree C. Heller,Richard Heller +1 more
TL;DR: This minireview is limited in scope to the most recent studies using in vivo EP for delivery of plasmid DNA, and focuses on those studies that demonstrated a clear therapeutic response with the potential to be used clinically.
Journal ArticleDOI
Electroporation for the delivery of DNA-based vaccines and immunotherapeutics: current clinical developments.
TL;DR: Electroporation has been used in basic research for the past 25 years to aid in the transfer of DNA into cells in vitro and can be successfully used in many species, including humans.
Journal ArticleDOI
Phenotypic and Functional Analysis of Dendritic Cells and Clinical Outcome in Patients With High-Risk Melanoma Treated With Adjuvant Granulocyte Macrophage Colony-Stimulating Factor
Adil Daud,Noweeda Mirza,Brianna Lenox,S. Andrews,P. Urbas,Gui X. Gao,Ji-Hyun Lee,Vernon K. Sondak,Adam I. Riker,Ronald C. DeConti,Dmitry I. Gabrilovich +10 more
TL;DR: Granulocyte macrophage colony-stimulating factor can induce differentiation of dendritic cells (DCs) in preclinical models and greater increase of DCs was associated with remission or delayed recurrence, and the prolonged overall survival observed warrants further exploration.
Journal ArticleDOI
Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo.
Loree C. Heller,Mark J. Jaroszeski,Domenico Coppola,C Pottinger,Richard Gilbert,Richard Heller +5 more
TL;DR: The successful delivery of plasmids encoding reporter genes to rat hepatocellular carcinomas by in vivo electroporation is demonstrated.