Journal ArticleDOI
Electrorotation of lymphocytes—The influence of membrane events and nucleus
TLDR
Electrorotation allows the distinction between successfully stimulated lymphocytes and unstimulated cells after application of concanavalin A and detects an enhanced cell membrane conductivity for the whole cell population immediately after the addition of mitogen.Abstract:
Electrorotation--the spin of cells in rotating high frequency electric fields--has been used to investigate properties of human peripheral blood lymphocytes. The rotation spectra of lymphocytes deviate from those of single shell spheres. The deviations are caused by the electrical properties of the nucleus in the cell interior. Electrorotation allows the distinction between successfully stimulated lymphocytes and unstimulated cells after application of concanavalin A. Notwithstanding the fact that only a proportion of the cells will be mitogenically stimulated we detected an enhanced cell membrane conductivity for the whole cell population immediately after the addition of mitogen.read more
Citations
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Journal ArticleDOI
Single cell dielectric spectroscopy
TL;DR: In this article, a review of single cell dielectric spectroscopy methods is presented, including ac electrokinetic methods of dielectrophoresis and electrorotation.
Journal ArticleDOI
Dielectric properties of human leukocyte subpopulations determined by electrorotation as a cell separation criterion
TL;DR: The data reveal that dielectrophoretic cell sorters should have the ability to discriminate between, and to separate, leukocyte subpopulations under appropriate conditions.
Journal ArticleDOI
Dielectrophoresis-based sample handling in general-purpose programmable diagnostic instruments
TL;DR: The concept of an integrated programmable general-purpose sample analysis processor (GSAP) architecture where raw samples are routed to separation and analysis functional blocks contained within a single device is presented.
Journal ArticleDOI
Time domain dielectric spectroscopy study of human cells. II. Normal and malignant white blood cells.
TL;DR: The dielectric permittivity, capacitance and conductivity values of cell membranes are higher for normal lymphocytes than for the malignant ones and the difference of the same parameters for normal B and T-cells is discussed.
Journal ArticleDOI
Study of normal and malignant white blood cells by time domain dielectric spectroscopy
TL;DR: It was shown that dielectric permittivity, capacitance and conductivity values of the cell membrane are higher for normal lymphocytes in comparison with malignant ones.
References
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Journal ArticleDOI
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TL;DR: The permeability of the amphibian oocyte nuclear envelope in situ has been determined for three tritiated dextrans and the envelope is a sieve restricting molecular movement between the cytoplasm and nucleus.
Journal ArticleDOI
A common sequence of calcium and pH signals in the mitogenic stimulation of eukaryotic cells
T R Hesketh,John P. Moore,Jonathan D.H. Morris,Michael V. Taylor,John H. Rogers,Gerry A. Smith,James C. Metcalfe +6 more
TL;DR: It is shown that a common sequence of [Ca]i and pHi responses occurs in both quiescent mouse thymocytes and Swiss 3T3 fibroblasts stimulated by appropriate mitogens, and these ionic responses are obligatory for progression to DNA synthesis by the normal pathway after fertilization.
Book
Interactions between electromagnetic fields and cells
TL;DR: In this paper, the biological targets of electromagnetic exposure at the membrane and nuclear level are reviewed and the effects of electromagnetic fields on the interaction between ligands and their binding sites are discussed.
Journal ArticleDOI
Time sequence of nuclear pore formation in phytohemagglutinin-stimulated lymphocytes and in hela cells during the cell cycle
Gerd G. Maul,Helmut M. Maul,Joseph E. Scogna,Michael W. Lieberman,Gary S. Stein,Betty Yee Li Hsu,Thaddeus W. Borun +6 more
TL;DR: The time sequence of nuclear pore frequency changes was determined for phytohemagglutinin (PHA)-stimulated human lymphocytes and for HeLa S-3 cells during the cell cycle, leading to the rather speculative possibility that thenuclear pore might be the site of initiation and/or replication of DNA as well as the siteof nucleocytoplasmic exchange.
Journal ArticleDOI
Rotating-field-induced rotation and measurement of the membrane capacitance of single mesophyll cells of Avena sativa [oat]
W.M. Arnold,U. Zimmermann +1 more
TL;DR: In this paper, a planar, homogeneous rotating field has been observed to induce single cells to spin at a frequency of 20-40 kHz (conductivity of the external mannitol solution 6x 10-5 Ω-1 cm-1).