Journal ArticleDOI
Enantiomeric separation of oxomemazine in rabbit plasma by ultra-fast LC and application in a stereoselective pharmacokinetic study.
Prachi P Raikar,Bannimath Gurupadayya,Surulivel Rajan,Sai Geetha Koganti,Peddaguravagari Mounika +4 more
TLDR
The findings revealed that stereospecific distribution of oxomemazine enantiomers does not change significantly, and the ultra-fast LC technique with an amylose chiral column was used to establish a new bioanalytical method.Abstract:
Aim: Ultra-fast LC was used to establish a new bioanalytical method for enantiomeric separation of oxomemazine. Methods: The proposed study was carried out using the ultra-fast LC technique with an amylose chiral column. The bioanalytical approach was used in rabbit plasma following US FDA regulations and then extended to oxomemazine enantiomeric separation using metronidazole as the internal standard. Results: The retention times of (R)-oxomemazine, (S)-oxomemazine and the internal standard were found to be 9.511, 10.712 and 6.503 min, respectively. Within-run and between-run precision (percent relative standard deviation) was found to be in the range of 0.018-0.102% for (R)-oxomemazine and 0.028-0.675% for (S)-oxomemazine, whereas accuracy (%) was found to be in the range of 95.971-99.720% for (R)-oxomemazine and 97.199-103.921% for (S)-oxomemazine. Conclusion: The findings revealed that stereospecific distribution of oxomemazine enantiomers does not change significantly.read more
References
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Journal ArticleDOI
Optimization strategies for HPLC enantioseparation of racemic drugs using polysaccharides and macrocyclic glycopeptide antibiotic chiral stationary phases
Hassan Y. Aboul-Enein,Imran Ali +1 more
TL;DR: This review discusses the optimization of HPLC conditions for the chiral resolution of racemic drugs on polysaccharides and macrocyclic glycopeptide antibiotic chiral stationary phases (CSPs).
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Chiral Analysis of Ibuprofen Residues in Water and Sediment
TL;DR: In this paper, the presence of ibuprofen, the third most popular clinically used drug in the world, in water resources, its enantiomeric degradation, and the monitoring of chiral ratio of enantiomers and its degradation products.
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Advances in chiral separations of small peptides by capillary electrophoresis and chromatography
TL;DR: Efforts are made to explain the chiral recognition mechanisms, which will be helpful in understanding existing and developing new stereoselective analyses and the demand of enantiomeric resolution of all naturally occurring and synthetic peptides is concluded.
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Enantioselective Toxicity and Carcinogenesis
TL;DR: In a non-chiral environment, the enantiomers of a racemate possess the same physico-chemical properties but in the biological systems they possess different activities, so the knowledge of the stereoselective metabolisms of the chiral pollutants may be useful for the treatment of cancer and other diseases.
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Role of racemization in optically active drugs development
TL;DR: In vitro and in vivo racemization of optically active drugs is described, considering the effect of different variables i.e. pH, temperature, concentration of the drug, ionic concentration, etc.