Journal ArticleDOI
Enhanced elution of sperm from cotton swabs via enzymatic digestion for rape kit analysis.
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TLDR
Results indicate that cellulose‐digesting enzymes enhance the release of sperm and epithelial cells from a cotton swab over buffer alone, providing for efficient DNA analysis.Abstract:
This report describes development of a method for enhanced cell elution from cotton swabs. The method exploits an enzyme mixture for digestion of the cotton to remove intact cells, and can be utilized in conjunction with or to circumvent conventional differential extraction (DE). Samples digested with Aspergillus niger cellulase yielded sperm cell recoveries (18 ± 3.5%) similar to conventional DE buffer (23 ± 7.8%) while providing intact epithelial cells. Storage time affected the concentration of enzyme required for optimal sperm cell recovery, with longer times requiring increased cellulase concentrations. Cellulase from A. niger yielded a twofold enhancement in sperm cell elution over buffer alone, and preliminary testing of higher activity cellulases from Trichoderma reesei and Trichoderma viride showed even greater enhancement. These results indicate that cellulose-digesting enzymes enhance the release of sperm and epithelial cells from a cotton swab over buffer alone, providing for efficient DNA analysis.read more
Citations
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Journal ArticleDOI
Bioconversion of municipal solid waste to glucose for bio-ethanol production
TL;DR: The highest glucose yield was obtained with a pre-hydrolysis treatment consisting of H2SO4 at 1% concentration, followed by steam treatment at 121°C, and enzymatic hydrolysis with Trichoderma viride at 60 FPU g−1 substrate.
Patent
Nucleic acid purification
Richard F. Selden,Eugene Tan +1 more
TL;DR: In this article, a self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, comprises at least one input, and a drive mechanism on said instrument for driving said liquid purification reagent, through a microfluidic element and a purification matrix, wherein the only inputs to said apparatus are via said chamber and said drive mechanism.
Journal ArticleDOI
Post-coital vaginal sampling with nylon flocked swabs improves DNA typing.
TL;DR: The results imply that the use of nylon flocked swabs for vaginal sampling will improve microscopic analysis and DNA typing in the medical forensic investigation of sexual assault cases.
Journal ArticleDOI
Differential DNA extraction of challenging simulated sexual-assault samples: a Swiss collaborative study
Séverine Vuichard,Urs V. Borer,Michel Bottinelli,Christian Cossu,Naseem Malik,Verena Meier,Christian Gehrig,Andrea Sulzer,Marie Laure Morerod,Vincent Castella +9 more
TL;DR: The recovery of male and female DNA was highly variable, depending on the laboratory involved, and an experimental design similar to the one used in this study may be of assistance for local protocol testing and improvement.
Patent
Expanded beam fiber optic connection system
TL;DR: In this paper, an expanded beam fiber optic connection system is presented, which overcomes the problems associated with known expanded beam DF connection systems. But the configuration of the expanded beam termini provides several benefits, such as reducing the number of components for the two types of fiber optic connections and reducing manufacturing costs.
References
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Micro Total Analysis Systems. 1. Introduction, Theory, and Technology
TL;DR: In this article, the authors present a review of the book.http://www.reviewreviews.com/reviews/book-reviews-of-the-book
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Micro total analysis systems. 2. Analytical standard operations and applications.
TL;DR: This second part of the review of microfluidic system preparation will cover a number of standard operations as well as some biological applications of micro total analysis systems.
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Forensic application of DNA 'fingerprints'.
TL;DR: It is shown that this technique can be used for forensic purposes; DNA of high relative molecular mass (Mr) can be isolated from 4-yr-old bloodstains and semen stains made on cotton cloth and digested to produce DNA fingerprints suitable for individual identification.
Journal ArticleDOI
Single-molecule DNA amplification and analysis in an integrated microfluidic device.
TL;DR: This device demonstrates the most sensitive PCR possible in a microfabricated device and will also facilitate single-cell and single-molecule studies to expose the genetic variation underlying ensemble sequence and expression averages.
Journal ArticleDOI
Integrated system for rapid PCR-based DNA analysis in microfluidic devices.
Julia Khandurina,Timothy E. McKnight,Stephen C. Jacobson,Larry C. Waters,Robert S. Foote,J. Michael Ramsey +5 more
TL;DR: An integrated system for rapid PCR-based analysis on a microchip has been demonstrated and an on-chip DNA concentration technique has been incorporated into the system to further reduce analysis time by decreasing the number of thermal cycles required.