Journal ArticleDOI
Evaluation of a commercial integrating sphere setup for the determination of absolute photoluminescence quantum yields of dilute dye solutions.
Christian Würth,Cornelia Lochmann,Monika Spieles,Jutta Pauli,Katrin Hoffmann,Tanja Schüttrigkeit,Thomas Franzl,Ute Resch-Genger +7 more
TLDR
Evaluating the performance and sources of uncertainty of a simple commercial integrating sphere setup with dilute solutions of two of the best characterized fluorescence quantum yield standards, quinine sulfate dihydrate and rhodamine 101, strongly differing in spectral overlap between absorption and emission.Abstract:
The commercial availability of stand-alone setups for the determination of absolute photoluminescence quantum yields (pf) in conjunction with the increasing use of integrating sphere accessories for spectrofluorometers is expected to have a considerable influence not only on the characterization of chromophore systems for use in optical and opto-electronic devices, but also on the determination of this key parameter for (bio)analytically relevant dyes and functional luminophores. Despite the huge potential of systems measuring absolute pf values and the renewed interest in dependable data, evaluated protocols for even the most elementary case, the determination of the fluorescence quantum yield of transparent dilute solutions of small organic dyes with integrating sphere methods, are still missing. This encouraged us to evaluate the performance and sources of uncertainty of a simple commercial integrating sphere setup with dilute solutions of two of the best characterized fluorescence quantum yield standards, quinine sulfate dihydrate and rhodamine 101, strongly differing in spectral overlap between absorption and emission. Special attention is dedicated to illustrate common pitfalls of this approach, thereby deriving simple procedures to minimize measurement uncertainties and improve the comparability of data for the broad community of users of fluorescence techniques.read more
Citations
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Journal ArticleDOI
Relative and absolute determination of fluorescence quantum yields of transparent samples
TL;DR: This protocol describes procedures for relative and absolute determinations of Φf values of fluorophores in transparent solution using optical methods, and introduces a series of eight candidate quantum yield standards for the wavelength region of ∼350–950 nm.
Journal ArticleDOI
Comparison of Methods and Achievable Uncertainties for the Relative and Absolute Measurement of Photoluminescence Quantum Yields
TL;DR: This work compares two relative and one absolute fluorometric method for the determination of the fluorescence quantum yields of quinine sulfate dihydrate, coumarin 153, fluorescein, rhodamine 6G, and rhodamines 101, thereby providing evaluated standard operation procedures for Φ(f) measurements and, simultaneously, a set of assessed Φ (f) standards.
Journal ArticleDOI
Particle-Size-Dependent Förster Resonance Energy Transfer from Upconversion Nanoparticles to Organic Dyes
Verena Muhr,Christian Würth,Marco Kraft,Markus Buchner,Antje J. Baeumner,Ute Resch-Genger,Thomas Hirsch +6 more
TL;DR: Time-resolved studies of the upconversion luminescence of the UCNP donor revealed a considerable shortening of the donor lifetime as a clear hint for Förster resonance energy transfer (FRET).
Journal ArticleDOI
Determination of the photoluminescence quantum yield of dilute dye solutions (IUPAC Technical Report)
Ute Resch-Genger,Knut Rurack +1 more
TL;DR: In this article, a procedure for the determination of photoluminescence quantum yields with opti- cal methods is presented, and challenges associated with these measurements are discussed, with special emphasis dedicated to relative measurements of fluorescent (i.e., short excited state lifetime), transparent, dilute dye solutions in conventional cuvettes.
Journal ArticleDOI
Determination of the absolute fluorescence quantum yield of rhodamine 6G with optical and photoacoustic methods--providing the basis for fluorescence quantum yield standards.
Christian Würth,Martín G. González,Reinhard Niessner,Ulrich Panne,Christoph Haisch,Ute Resch Genger +5 more
TL;DR: This systematic comparison was performed in ethanol and in water employing different concentrations of R6G and experimental advantages and systematic sources of variation were identified for both methods.
References
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Journal ArticleDOI
Measurement of photoluminescence quantum yields. Review
Glenn A. Crosby,James N. Demas +1 more
BookDOI
Molecular fluorescence : principles and applications
TL;DR: In this article, the effects of intermolecular photophysical processes on fluorescence emission are discussed and an analysis of the effect of polarity of fluorescence emissions is presented.
Journal ArticleDOI
Quantum dots versus organic dyes as fluorescent labels
TL;DR: This work compares and evaluates the differences in physicochemical properties of common fluorescent labels, focusing on traditional organic dyes and QDs, to provide a better understanding of the advantages and limitations of both classes of chromophores.
Journal ArticleDOI
An improved experimental determination of external photoluminescence quantum efficiency
Journal ArticleDOI
Fluorescence quantum yields of some rhodamine dyes
R.F. Kubin,A.N. Fletcher +1 more
TL;DR: In this article, the quantum yields of seven rhodamine dyes were measured relative to quinine sulfate dihydrate (QSH) in 1.0 N H 2 SO 4.