Exploiting the triple response of Arabidopsis to identify ethylene-related mutants.
Plinio Guzmán,Joseph R. Ecker +1 more
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Alterations in the response of dark-grown seedlings to ethylene (the "triple response") were used to isolate a collection of ethylene-related mutants in Arabidopsis thaliana and should prove to be useful tools for dissecting the mode of Ethylene action in plants.Abstract:
Alterations in the response of dark-grown seedlings to ethylene (the "triple response") were used to isolate a collection of ethylene-related mutants in Arabidopsis thaliana. Mutants displaying a constitutive response (eto1) were found to produce at least 40 times more ethylene than the wild type. The morphological defects in etiolated eto1-1 seedlings reverted to wild type under conditions in which ethylene biosynthesis or ethylene action were inhibited. Mutants that failed to display the apical hook in the absence of ethylene (his1) exhibited reduced ethylene production. In the presence of exogenous ethylene, hypocotyl and root of etiolated his1-1 seedlings were inhibited in elongation but no apical hook was observed. Mutants that were insensitive to ethylene (ein1 and ein2) produced increased amounts of ethylene, displayed hormone insensitivity in both hypocotyl and root responses, and showed an apical hook. Each of the "triple response" mutants has an effect on the shape of the seedling and on the production of the hormone. These mutants should prove to be useful tools for dissecting the mode of ethylene action in plants.read more
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Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
Jose M. Alonso,Anna Stepanova,Thomas J. Leisse,Christopher J. Kim,Huaming Chen,Paul Shinn,Denise K. Stevenson,Justin Zimmerman,Pascual Barajas,Rosa Cheuk,Carmelita Gadrinab,Collen Heller,Albert Jeske,Eric Koesema,Cristina C. Meyers,Holly Parker,Lance Prednis,Yasser Ansari,Nathan Choy,Hashim Deen,Michael Geralt,Nisha Hazari,Emily Hom,Meagan Karnes,Celene Mulholland,Ral Ndubaku,Ian Thomas Schmidt,Plinio Guzmán,Laura Aguilar-Henonin,Markus Schmid,Detlef Weigel,David E. Carter,Trudy Marchand,Eddy Risseeuw,Debra Brogden,Albana Zeko,William L. Crosby,Charles C. Berry,Joseph R. Ecker +38 more
TL;DR: Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels, and insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.
Journal ArticleDOI
CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases
TL;DR: A recessive Arabidopsis mutant, ctr1, that constitutively exhibits seedling and adult phenotypes observed in plants treated with the plant hormone ethylene is isolated and the DNA sequences of four mutant alleles were determined.
Journal ArticleDOI
Ethylene biosynthesis and signaling networks.
TL;DR: The plant hormone ethylene is involved in many aspects of the plant life cycle, including seed germination, root hair development, root nodulation, flower senescence, and more.
Journal ArticleDOI
Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators
TL;DR: An early step in ethylene signal transduction in plants may involve transfer of phosphate as in prokaryotic two-component systems.
Journal ArticleDOI
Growth Stage –Based Phenotypic Analysis of Arabidopsis: A Model for High Throughput Functional Genomics in Plants
Douglas Boyes,Adel Zayed,Robert Ascenzi,Amy J. McCaskill,Neil Hoffman,Keith R. Davis,Jörn Görlach +6 more
TL;DR: Analysis of the development of wild-type Columbia (Col-0) plants and selected mutants are presented to illustrate a framework methodology that can be used to identify and interpret phenotypic differences in plants resulting from genetic variation and/or environmental stress.
References
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Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
Ethylene biosynthesis and its regulation in higher plants
Shang Fa Yang,Neil E. Hoffman +1 more
TL;DR: The pathways of Ethylene Biosynthesis, Regulation in Ripening Fruits and Senescing Flowers and Regulation by Light and Carbon Dioxide are traced.
Book
Ethylene in plant biology
TL;DR: This book discusses Ethylene Analysis and Properties of the Gas, the Role of Ethylene in Agriculture, and Roles and Physiological Effects ofEthylene in Plant Physiology: Dormancy, Growth and Development.
Ethylene in Plant Biology
TL;DR: This book discusses Ethylene Analysis and Properties of the Gas, the Role of Ethylene in Agriculture, and Roles and Physiological Effects ofEthylene in Plant Physiology: Dormancy, Growth and Development.