Journal ArticleDOI
Expression and activity of matrix metalloproteinases in the uterus of bitches after spontaneous and induced abortion.
TLDR
The blockade of the biological progesterone effect was associated with an increase in activity of both collagenases, and at interplacental sites, the amount of active collagenases in the myometrium was significantly lower in the control group.Abstract:
Contents
Aim of this study was to determine the intrauterine activity of matrix metalloproteinases (MMP)-2 and -9 after cessation of the local effect of progesterone. For this purpose, pregnancy was terminated in 10 bitches at mid-gestation with the progesterone receptor antagonist aglepristone (10 mg/kg body weight, sc, Alizine®; Virbac, France) at two subsequent days (group IRA = induced resorption/abortion). The IRA group was divided into two subgroups (Group I, n = 5, days 25–35 of pregnancy; group II, n = 5, days 36–45). Five further bitches were introduced with beginning abortion (group SRA = spontaneous resorption/abortion). Seven healthy bitches between day 25 and 45 of gestation served as controls. After ovariohysterectomy at the end of abortion and between days 25 and 45 of gestation, respectively, the distribution and activity of collagenases were investigated by immunohistochemistry and gelatin zymography. At placental sites, MMP-2 activity in the endometrium was significantly lower in IRA groups than in the SRA group (33.7 ± 11.8% and 39.3 ± 5.4% vs 52.2 ± 10.2%, p < 0.05); however, MMP-2 expression was lowest in the control group (control: 21.4 ± 6.3%; p < 0.01) and similarly in the myometrium (controls: 13.1 ± 2.5%; p < 0.05). MMP-9 activity was also lower in the endometrium and myometrium of the control group in comparison to SRA and IRA groups (11.8 ± 3.2%; p < 0.01 and 28.4 ± 32.8%; p < 0.05). At interplacental sites, the amount of active collagenases in the myometrium was significantly lower in the control group. It is concluded that the blockade of the biological progesterone effect was associated with an increase in activity of both collagenases.read more
Citations
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Journal ArticleDOI
Circulating matrix metalloproteinase MMP-9 and MMP-2/TIMP-2 complex are associated with spontaneous early pregnancy failure
Ritva Nissi,Anne Talvensaari-Mattila,Vesa Kotila,Maarit Niinimäki,Ilkka Y. Järvelä,Taina Turpeenniemi-Hujanen +5 more
TL;DR: Human placentation is mediated by fetal trophoblastic cells that invade the maternal uterine endometrium that require a precisely regulated secretion of specific proteolytic enzymes able to degrade the endometrial basement membrane and extracellular matrix.
Journal ArticleDOI
Early canine pregnancy--a battle for successful growth and angiogenesis.
TL;DR: The work investigating immunological changes in pregnant bitches has elucidated aspects of the complex physiology of implantation but raises important questions about the mechanisms involved.
Journal ArticleDOI
The Role of Progestogens in Regulating Matrix Metalloproteinase Activity in Macrophages and Microglial Cells
TL;DR: The results demonstrate that the effects of PG, PG metabolites and MPA on MMP-9 enzyme activity differ across vascular and brain cells when administered alone or in combination with E2 which could have important mechanistic implications for hormone therapy.
Journal ArticleDOI
Progesterone receptor blockers: historical perspective, mode of function and insights into clinical and scientific applications
TL;DR: Besides their clinical application, antigestagens are used in research for investigating P4-dependent physiological and pathological processes and do not require the presence of the natural ligand for their action.
Journal ArticleDOI
Differential Expression of EGFR-1, MMP-3, and MMP-9 in Spontaneous Abortions, Induced Abortions, and Tubal Pregnancies.
Mahi Balci,Gulhan Ozdemir +1 more
TL;DR: Higher EGfr-1 expression in the decidual tissue of spontaneous abortion specimens suggests that EGFR-1 triggers the migration of extravillous trophoblasts, leading to their destructive invasion.
References
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