Journal ArticleDOI
Fast and Reliable Automated Synthesis of RNA and Partially 2′-O- Protected Precursors (`Caged RNA') Based on Two Novel, Orthogonal 2′-O-Protecting Groups, Preliminary Communication
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TLDR
It could be demonstrated that the presence of one (R)-npeom group within a RNA strand has only a minor influence on the pairing properties of corresponding duplexes.Abstract:
Two sets of RNA phosphoramidites, carrying the (fluoride-labile) 2'-O-[(triisopropylsilyl)oxy]methyl (tom) group and the (photolabile) [(R)-1-(2-nitrophenyl)ethoxy]methyl ((R)-npeom) group, were prepd. The two protecting groups were completely orthogonal to each other. Three ribozyme-substrate constructs, protected each by a (R)-npeom group, were synthesized; on photolysis, efficient cleavage of this remaining protecting group occurred. It could be demonstrated that the presence of one (R)-npeom group within a RNA strand has only a minor influence on the pairing properties of corresponding duplexes. [on SciFinder (R)]read more
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Journal ArticleDOI
Biologically active molecules with a "light switch".
Günter Mayer,Alexander Heckel +1 more
TL;DR: This review summarizes new developments of the last five years and deals with "small molecules", proteins, and nucleic acids which can either be irreversibly activated with light (these compounds are referred to as "caged compounds") or reversibly switched between an active and an inactive state.
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Automated Chemical Oligosaccharide Synthesis: Novel Approach to Traditional Challenges.
TL;DR: The overarching goal of this review article is to present the advances that have been made at the interface of carbohydrate chemistry and automated technology.
Journal ArticleDOI
Biologisch aktive Moleküle mit “Lichtschalter”
Günter Mayer,Alexander Heckel +1 more
TL;DR: In this article, aktive Verbindungen, die auf Licht reagieren, bieten experimentelle Moglichkeiten, which sonst nur sehr schwierig zu realisieren sind.
Journal ArticleDOI
Light regulation of aptamer activity: an anti-thrombin aptamer with caged thymidine nucleobases.
Alexander Heckel,Günter Mayer +1 more
TL;DR: "Caged" derivatives of a 15 nucleotide ssDNA anti-thrombin aptamer have been synthesized in which thymidine nucleotides are modified with photolabile protecting groups, yielding the unmodified active aptamer.
Journal ArticleDOI
Regulating gene expression with light-activated oligonucleotides
Xinjing Tang,Ivan J. Dmochowski +1 more
TL;DR: In the near future, the authors can expect photoresponsive DNA and RNA molecules that are relatively non-toxic, nuclease-resistant, and maintain their specificity and activity in vivo.