Journal ArticleDOI
Feasibility Studies on Vitrification of Intact Zebrafish (Brachydanio rerio) Embryos
Tiantian Zhang,David M. Rawson +1 more
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TLDR
The attempts at vitrification of zebrafish embryos failed to result in embryo viability, and the low degree of cryoprotectant permeability of the embryos is considered to be directly responsible for embryo injury during vitrification procedures.About:
This article is published in Cryobiology.The article was published on 1996-02-01. It has received 100 citations till now. The article focuses on the topics: Cryoprotectant & Vitrification.read more
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Journal ArticleDOI
Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification
Joseph Saragusty,Amir Arav +1 more
TL;DR: The major strides and obstacles in human and other vertebrate oocyte and embryo cryopreservation will be reviewed here.
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Field emission scanning electron microscopy and transmission electron microscopy studies of the chorion, plasma membrane and syncytial layers of the gastrula‐stage embryo of the zebrafish Brachydanio rerio: a consideration of the structural and functional relationships with respect to cryoprotectant penetration
TL;DR: The significance of the structural detail shown in these studies is considered in the light of the difficulties experienced in cryopreservation of the embryo resulting from the inability of achieving cryoprotectant penetration of the yolk mass.
Journal ArticleDOI
Characterization of a Major Permeability Barrier in the Zebrafish Embryo
TL;DR: Zebrafish embryos have two major compartments, the blastoderm and the yolk, which is surrounded by the multinucleated yolk syncytial layer (YSL), and the water and cryoprotectant permeability in these compartments was determined using two methods.
Journal ArticleDOI
Chill Sensitivity and Cryoprotectant Permeability of Dechorionated Zebrafish Embryos,Brachydanio rerio
TL;DR: It is concluded that the complex nature of the zebrafish embryo reduces the effectiveness and predictive value of light microscopical measurements for cryoprotectant permeability studies.
Journal ArticleDOI
Interaction of the sugars trehalose, maltose and glucose with a phospholipid bilayer: a comparative molecular dynamics study.
TL;DR: Molecular dynamics simulations are used to investigate the interaction of the sugars trehalose, maltose, and glucose with a phospholipid bilayer at atomic resolution, finding that at high temperature, the three sugars are able to prevent the thermal disruption of the bilayer.
Related Papers (5)
Studies on Chilling Sensitivity of Zebrafish (Brachydanio rerio) Embryos
Tiantian Zhang,David M. Rawson +1 more