Journal ArticleDOI
Fermentation of biodiesel-derived glycerol by Bacillus amyloliquefaciens: effects of co-substrates on 2,3-butanediol production.
TLDR
Results are the highest report for 2,3-BD fermentation from biodiesel-derived glycerol in the presence of beet molasses as a co-substrate and simultaneously reduced the duration of fermentation.Abstract:
Cultivation in glycerol instead of sugars inhibits 2,3-butanediol (2,3-BD) production by Bacillus amyloliquefaciens. In this study, we report that B. amyloliquefaciens readily produces 2,3-BD from biodiesel-derived glycerol in the presence of beet molasses as a co-substrate. Unexpectedly, the molasses stimulated 2,3-BD production and simultaneously reduced the duration of fermentation. Productivity of 2,3-BD was enhanced at the start of fermentation, and yields increased under continuous molasses supply. Subsequently, 2,3-BD production in molasses-supplemented fed-batch culture was observed. Prior to inoculation of fed-batch fermentation culture, 15 g/l of molasses was added to the bioreactor. After 6 h of incubation, the bioreactor was fed with a solution containing 80 % glycerol and 15 % molasses. The 2,3-BD concentration, yield, and productivity significantly improved, reaching 83.3 g/l, 0.42 g/g, and 0.87 g/l·h, respectively. To our knowledge, these results are the highest report for 2,3-BD fermentation from biodiesel-derived glycerol.read more
Citations
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Journal ArticleDOI
Value-added processing of crude glycerol into chemicals and polymers.
TL;DR: Recent advances of biological and chemical technologies for value-added processing of crude glycerol into chemicals and polymers are reviewed, and strategies for addressing production challenges are provided.
Journal ArticleDOI
High production of 2,3-butanediol from biodiesel-derived crude glycerol by metabolically engineered Klebsiella oxytoca M1.
Sukhyeong Cho,Taeyeon Kim,Taeyeon Kim,Han Min Woo,Han Min Woo,Yunje Kim,Jinwon Lee,Youngsoon Um,Youngsoon Um +8 more
TL;DR: 2,3-BDO production from glycerol was dramatically enhanced by disruption of the pduC and ldhA genes in K. oxytoca M3, demonstrating the feasibility of economic industrial 2, 3- BDO production using crude Glycerol.
Journal ArticleDOI
Metabolic engineering strategies for acetoin and 2,3-butanediol production: advances and prospects.
TL;DR: This review summarizes metabolic engineering strategies for improvement of microbial acetoin and 2,3-BD production and proposes enhancements to current acetoin-2,3,BD production strategies, by offering a metabolic engineering approach that is guided by systems biology and synthetic biology.
Journal ArticleDOI
Enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in Bacillus amyloliquefaciens
TL;DR: This is the highest reported 2, 3-BD production using biodiesel-derived glycerol as substrate, and this designed B. amyloliquefaciens should be an excellent candidate for producing 2,3-BD on an industrial scale.
Journal ArticleDOI
Enhanced acetoin production by Bacillus amyloliquefaciens through improved acetoin tolerance
Qiuling Luo,Jing Wu,Minchen Wu +2 more
TL;DR: An acetoin-tolerant mutant E-11 was screened by using adaptive evolution with acetoin stress as the selection pressure to enhance the resistance of B. amyloliquefaciens to acetoin and exhibited superior fermentation performance.
References
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Journal ArticleDOI
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