Journal ArticleDOI
Film and pore diffusion studies with immobilized glucose oxidase
B. J. Rovito,J. R. Kittrell +1 more
TLDR
It appears that immobilized enzymes free of internal diffusional restrictions generally require the use of supports with pore sizes larger than those currently available in porous glass.Abstract:
Quantitative studies are reported of external film and internal pore diffusion effects for glucose oxidase immobilized on porous glass in a continuous, tubular, packed bed reactor. The McCune and Wilhelm model for external film diffusion is shown to describe these data over a particle Reynolds number range from 0.2 to 25. The Thiele analysis provides a good description of internal pore diffusion resistances. It appears that immobilized enzymes free of internal diffusional restrictions generally require the use of supports with pore sizes larger than those currently available in porous glass.read more
Citations
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Journal ArticleDOI
The immobilization of whole cells: Engineering principles
TL;DR: Techniques which have been used to immobilize whole cells include adsorption, aggregation, confinement and entrapment, and many more have been proposed.
Book ChapterDOI
[29] Kinetic behavior of immobilized enzyme systems
TL;DR: This chapter discusses the kinetic behavior of immobilized enzyme systems, which can be controlled by both microenvironmental and mass-transfer effects.
Journal ArticleDOI
Combined effects of external mass transfer and biodegradation rates on removal of phenol by immobilized Ralstonia eutropha in a packed bed reactor.
Ozlem Tepe,Arzu Y. Dursun +1 more
TL;DR: The intrinsic first-order biodegradation rate constants and the external mass transfer coefficients were calculated and the combined effects of these rates on the observed first- order biodegrading rate constants were investigated.
Book ChapterDOI
Diffusion and Kinetics with Immobilized Enzymes
Jean-Marc Engasser,Csaba Horváth +1 more
TL;DR: This chapter discusses the analysis of the kinetic behavior of enzymes entrapped in or bound to membranes or other supports, as can be observed from macroscopic measurements.
Journal ArticleDOI
Influence of intraparticle diffuisional limitation on the observed kinetics of immobilized enzymes and on catalyst design
TL;DR: Numerical solutions to the equations describing simultaneous mass transfer and enzymic reaction within porous spherical particles have been used to examine the effect of enzyme content and other parameters on the kinetic behavior of immobilized enzymes.
References
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Book
Chemical Reaction Engineering
TL;DR: An overview of Chemical Reaction Engineering is presented, followed by an introduction to Reactor Design, and a discussion of the Dispersion Model.
Journal ArticleDOI
Mass Transfer (Absorption) Coefficients Prediction from Data on Heat Transfer and Fluid Friction
Thomas H. Chilton,A. P. Colburn +1 more
Journal ArticleDOI
The kinetics of carboxymethylcellulose-ficin in packed beds
Lilly,WE Hornby,EM Crook +2 more
TL;DR: The kinetics of the hydrolysis of benzoylarginine ethyl ester in packed columns of CM-cellulose-70-ficin and CM-cells-90- ficin were studied and the apparent Michaelis constant was calculated and shown to be dependent on the flow rate at low rates of perfusion through the columns.