Journal ArticleDOI
Effect of internal diffusion in heterogeneous enzyme systems: evaluation of true kinetic parameters and substrate diffusivity.
Jean-Marc Engasser,Csaba Horváth +1 more
TLDR
A graphical method is proposed to determine KM and Vmax‴ as well as the substrate diffusivity in the enzymic medium, since the conventional plots of enzyme kinetics do not yield straight lines under such conditions.About:
This article is published in Journal of Theoretical Biology.The article was published on 1973-11-05. It has received 132 citations till now. The article focuses on the topics: Immobilized enzyme & Reaction rate.read more
Citations
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Journal ArticleDOI
Enhanced Activity of Immobilized or Chemically Modified Enzymes
Yifei Zhang,Jun Ge,Zheng Liu +2 more
TL;DR: This work proposes that enhanced enzymatic activities can be attributed to the well-designed specific interactions between immobilization carriers (or chemical modifiers) and enzymes, substrates, or reaction media, and is applicable for both single- and multiple-enzyme systems.
Journal ArticleDOI
On the role of organized multienzyme systems in cellular metabolism: a general synthesis.
TL;DR: Throughout this article, vector quantities are denoted by use of bold type face and the symbol ▿ denotes the “gradient” operator and δ(r−r′) the three-dimensional Dirac delta function.
Book ChapterDOI
[29] Kinetic behavior of immobilized enzyme systems
TL;DR: This chapter discusses the kinetic behavior of immobilized enzyme systems, which can be controlled by both microenvironmental and mass-transfer effects.
Journal ArticleDOI
Mass transfer effects in fermentations using immobilized whole cells
TL;DR: In this paper, the authors examined the mass transfer limitations of mass transfer in the case of immobilized whole cells for fermentation and showed that they can be used to bring the understanding of heterogeneous catalysis.
Journal ArticleDOI
External and internal diffusion in heterogeneous enzymes systems.
Csaba Horváth,Jean-Marc Engasser +1 more
TL;DR: The intrusion of diffusion in heterogeneous enzyme reactions, which follow, is described and a graphical method is described for the evaluation of the reaction rate as a function of the surface concentration of the substrate from measured data.
References
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Journal ArticleDOI
A Note on the Kinetics of Enzyme Action
TL;DR: The equation of Michaelis and Menten [1913] has been applied with success by Kuhn [1924] and others to numerous cases of enzyme action and it is desirable to examine its theoretical basis.
Book
Quasilinearization and nonlinear boundary-value problems
Richard Bellman,Robert E. Kalaba +1 more
TL;DR: Quasilinearization and nonlinear boundary value problems as discussed by the authors, where the boundary value problem is formulated as a quadratic equation of the value of a boundary value.
Journal ArticleDOI
Multiphase catalysis. II. Hollow fiber catalysts
TL;DR: In this paper, a theory of diffusion control within hollow fiber catalysts is derived for three different types of coordinate geometries: Cartesian, cylindrical, and spherical, and effectiveness factors are calculated and formulas for reactant conversion in both a fixed-bed and a contimunuous-feed stirred-tank reactor are derived.
Journal ArticleDOI
Kinetic behavior at high enzyme concentrations. Magnitude of errors of Michelis-Menten and other approximations.
TL;DR: The errors of the Michaelis-Menten equation and three other approximations have been examined in relation to the true rate equation in which the depletion of free substrate by binding to the enzyme is taken into account.
Related Papers (5)
External and internal diffusion in heterogeneous enzymes systems.
Csaba Horváth,Jean-Marc Engasser +1 more