Q2. What are the common methods used to study biofilms?
Strategies adapted to the study of highly heterogeneous environments such as in vivo or promoter-trap based strategies (STM, IVET, RIVET) [29] or gene-targeted gfp fusions have been underused and will also certainly be helpful identifying genes only transiently expressed within biofilm sub-populations.
Q3. What is the role of genes in biofilms?
The transcriptome and proteome analyses performed on biofilms taken at different ages demonstrated that gene expression is changing along time.
Q4. Why is the biofilm gene expression pattern difficult to extract?
due to the absence of experimental gold standards, extracting a biofilm gene expression pattern from the available data is still difficult.
Q5. What is the role of stress in biofilms?
Membrane stress, triggered by bacteria-surface and bacteria-bacteria interactions, could therefore constitute anatural signal for the activation of several regulatory pathways that would promote stabilization and/or maturation of the biofilm.
Q6. What is the role of the biofilm in the biofilm?
Owing to the apparently minimal overlap between functions involved in biofilm formation by different bacteria, exploring the biofilm lifestyle could prove to be a case-by-case task for which global approaches show their limits.
Q7. What is the role of genes of unknown function in biofilms?
The prevalence of genes of unknown function in biofilm differentially expressed genesBiofilms are considered to be environments where new, or previously unrecognized,biological properties could be expressed.